{"doi":"10.1099/jmm.0.47498-0","title":"A comparative study of three different PCR assays for detection of Mycoplasma genitalium in urogenital specimens from men and women","abstract":"<jats:p>The aim of this study was to compare conventional 16S rRNA gene PCR, real-time 16S rRNA gene PCR and real-time <jats:italic>Mycoplasma genitalium</jats:italic> adhesin protein (MgPa) gene PCR as detection methods for <jats:italic>M. genitalium</jats:italic> infection. The study also determined the prevalence of <jats:italic>M. genitalium</jats:italic> in male and female patients attending a sexually transmitted infections clinic in a rural area in the west of Sweden. First void urine (FVU) and/or urethral swabs were collected from 381 men, and FVU and/or cervical swabs and/or urethral swabs were collected from 298 women. A total of 213 specimens were used in the PCR comparative study: 98 consecutively sampled specimens from patients enrolled in the prevalence study, 36 consecutively sampled specimens from patients with symptoms of urethritis and 79 specimens from patients positive for <jats:italic>M. genitalium</jats:italic> by real-time MgPa gene PCR in the prevalence study. A true-positive <jats:italic>M. genitalium</jats:italic> DNA specimen was defined as either a specimen positive in any two PCR assays or a specimen whose PCR product was verified by DNA sequencing. The prevalence of <jats:italic>M. genitalium</jats:italic> infection in men and women was 27/381 (7.1 %) and 23/298 (7.7 %), respectively. In the PCR comparative study, <jats:italic>M. genitalium</jats:italic> DNA was detected in 61/76 (80.3 %) of true-positive specimens by conventional 16S rRNA gene PCR, in 52/76 (68.4 %) by real-time 16S rRNA gene PCR and in 74/76 (97.4 %) by real-time MgPa gene PCR. Real-time MgPa gene PCR thus had higher sensitivity compared with conventional 16S rRNA gene PCR and had considerably increased sensitivity compared with real-time 16S rRNA gene PCR for detection of <jats:italic>M. genitalium</jats:italic> DNA. Real-time MgPa gene PCR is well suited for the clinical diagnosis of <jats:italic>M. genitalium</jats:italic>.</jats:p>","journal":"Journal of Medical Microbiology","year":2008,"id":659027,"datarank":0.6038027536102726,"base_score":4.02535169073515,"endowment":4.02535169073515,"self_citation_contribution":0.6038027536102726,"citation_network_contribution":0.0,"self_endowment_contribution":0.6038027536102726,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":55,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1720386,"name":"Margaretha Jurstrand","orcid":null,"position":1,"is_corresponding":false},{"id":1233793,"name":"Eva Johansson","orcid":null,"position":2,"is_corresponding":false},{"id":1720387,"name":"Elisabeth Wikander","orcid":null,"position":3,"is_corresponding":false},{"id":1720388,"name":"Anna Höög","orcid":null,"position":4,"is_corresponding":false},{"id":1720391,"name":"Thomas Ahlqvist","orcid":null,"position":5,"is_corresponding":false},{"id":1447195,"name":"Lars Falk","orcid":"0000-0002-1845-7418","position":6,"is_corresponding":false},{"id":411199,"name":"Jørgen Skov Jensen","orcid":"0000-0002-7464-7435","position":7,"is_corresponding":false},{"id":1720398,"name":"Hans Fredlund","orcid":null,"position":8,"is_corresponding":false},{"id":1720385,"name":"Andreas Edberg","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A comparative study of three different PCR assays for detection of Mycoplasma genitalium in urogenital specimens from men and women","abstract":"<jats:p>The aim of this study was to compare conventional 16S rRNA gene PCR, real-time 16S rRNA gene PCR and real-time <jats:italic>Mycoplasma genitalium</jats:italic> adhesin protein (MgPa) gene PCR as detection methods for <jats:italic>M. genitalium</jats:italic> infection. The study also determined the prevalence of <jats:italic>M. genitalium</jats:italic> in male and female patients attending a sexually transmitted infections clinic in a rural area in the west of Sweden. First void urine (FVU) and/or urethral swabs were collected from 381 men, and FVU and/or cervical swabs and/or urethral swabs were collected from 298 women. A total of 213 specimens were used in the PCR comparative study: 98 consecutively sampled specimens from patients enrolled in the prevalence study, 36 consecutively sampled specimens from patients with symptoms of urethritis and 79 specimens from patients positive for <jats:italic>M. genitalium</jats:italic> by real-time MgPa gene PCR in the prevalence study. A true-positive <jats:italic>M. genitalium</jats:italic> DNA specimen was defined as either a specimen positive in any two PCR assays or a specimen whose PCR product was verified by DNA sequencing. The prevalence of <jats:italic>M. genitalium</jats:italic> infection in men and women was 27/381 (7.1 %) and 23/298 (7.7 %), respectively. In the PCR comparative study, <jats:italic>M. genitalium</jats:italic> DNA was detected in 61/76 (80.3 %) of true-positive specimens by conventional 16S rRNA gene PCR, in 52/76 (68.4 %) by real-time 16S rRNA gene PCR and in 74/76 (97.4 %) by real-time MgPa gene PCR. Real-time MgPa gene PCR thus had higher sensitivity compared with conventional 16S rRNA gene PCR and had considerably increased sensitivity compared with real-time 16S rRNA gene PCR for detection of <jats:italic>M. genitalium</jats:italic> DNA. Real-time MgPa gene PCR is well suited for the clinical diagnosis of <jats:italic>M. genitalium</jats:italic>.</jats:p>","is_dataset_classified":null,"base_score":4.02535169073515,"endowment":4.02535169073515,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18287292","pmcid":null,"openalex_id":"https://openalex.org/W2075984439","authors":[],"funders":[],"total_grants":0,"fwci":4.0902,"citation_percentile":0.93674391,"influential_citations":0,"citation_trend":[{"year":2012,"count":5},{"year":2013,"count":6},{"year":2014,"count":3},{"year":2015,"count":3},{"year":2016,"count":4},{"year":2017,"count":2},{"year":2018,"count":5},{"year":2019,"count":1},{"year":2020,"count":3},{"year":2021,"count":1},{"year":2023,"count":1},{"year":2024,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.47498-0?crawler=true","host_type":"publisher"},{"url":"https://doi.org/10.1099/jmm.0.47498-0","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/18287292","host_type":"repository"}],"fields_of_study":["Microbial infections and disease research","Genital Health and Disease","Blood groups and transfusion","Adhesins, Bacterial","Adolescent","Adult","Aged","Aged, 80 and over","Cervix Uteri","Female","Humans","Male","Middle Aged","Mycoplasma Infections","Mycoplasma genitalium","Polymerase Chain Reaction","Prevalence","RNA, Ribosomal, 16S","Rural Population","Specimen Handling","Sweden","Urethra","Urine"],"mesh_terms":["Adolescent","Adult","Aged","Aged, 80 and over","Cervix Uteri","Female","Humans","Male","Middle Aged","Mycoplasma Infections","RNA, Ribosomal, 16S","Rural Population","Specimen Handling","Sweden","Urethra","Urine","Prevalence","Polymerase Chain Reaction","Adhesins, Bacterial","Mycoplasma genitalium"],"keywords":["Mycoplasma genitalium","16S ribosomal RNA","Urethritis","Polymerase chain reaction","Biology","Chlamydia trachomatis","Mycoplasma","Medicine","Gene","Gynecology","Microbiology","Virology","Internal medicine","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T06:01:36.850225Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}