{"doi":"10.1099/jmm.0.009977-0","title":"Real-time PCR detection of the mg219 gene of unknown function of Mycoplasma genitalium in men with and without non-gonococcal urethritis and their female partners in England","abstract":"<jats:p>Real-time PCR was employed to detect a region of the <jats:italic>Mycoplasma genitalium</jats:italic> \n                  <jats:italic>mg219</jats:italic> gene, a gene of unknown function, in clinical samples. Amplification of DNA and signal production from 15 other species of human mycoplasmas and 14 other bacteria and viruses did not occur. Using a panel of 208 genital and rectal samples, the sensitivity when compared to the modified <jats:italic>mgpa</jats:italic> gene (encoding the major surface protein MgPa) real-time PCR assay was found to be 100 % and the specificity of the assay 99.5 % with a positive predictive value of 80 % and a negative predictive value of 100 %. The <jats:italic>mg219</jats:italic> gene was found to be in all strains of <jats:italic>M. genitalium</jats:italic> and was highly conserved. <jats:italic>M. genitalium</jats:italic> was detected in 3.9 % (11/280, 95 % CI 2.1–6.9) of all male specimens, in 7.7 % (10/130, 95 % CI 4.1–13.7) of patients with non-gonococcal urethritis (NGU) and in 0.7 % (1/150, 95 % CI &lt;0.01–4.1) of patients without urethritis. The presence of <jats:italic>M. genitalium</jats:italic> was significantly associated with NGU (<jats:italic>P</jats:italic> ≤0.01; 95 % Cl 0.88–0.98) and non-chlamydial-non-gonococcal urethritis (<jats:italic>P</jats:italic>=0.0005; 95 % Cl 0.84–0.97).</jats:p>","journal":"Journal of Medical Microbiology","year":2009,"id":658378,"datarank":0.4943755299006494,"base_score":3.295836866004329,"endowment":3.295836866004329,"self_citation_contribution":0.4943755299006494,"citation_network_contribution":0.0,"self_endowment_contribution":0.4943755299006494,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":26,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1718681,"name":"Karen Jordan","orcid":null,"position":1,"is_corresponding":false},{"id":1718682,"name":"Tahir Ali","orcid":null,"position":2,"is_corresponding":false},{"id":1718683,"name":"Cathy Ison","orcid":null,"position":3,"is_corresponding":false},{"id":1718680,"name":"Victoria J. Chalker","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Real-time PCR detection of the mg219 gene of unknown function of Mycoplasma genitalium in men with and without non-gonococcal urethritis and their female partners in England","abstract":"<jats:p>Real-time PCR was employed to detect a region of the <jats:italic>Mycoplasma genitalium</jats:italic> \n                  <jats:italic>mg219</jats:italic> gene, a gene of unknown function, in clinical samples. Amplification of DNA and signal production from 15 other species of human mycoplasmas and 14 other bacteria and viruses did not occur. Using a panel of 208 genital and rectal samples, the sensitivity when compared to the modified <jats:italic>mgpa</jats:italic> gene (encoding the major surface protein MgPa) real-time PCR assay was found to be 100 % and the specificity of the assay 99.5 % with a positive predictive value of 80 % and a negative predictive value of 100 %. The <jats:italic>mg219</jats:italic> gene was found to be in all strains of <jats:italic>M. genitalium</jats:italic> and was highly conserved. <jats:italic>M. genitalium</jats:italic> was detected in 3.9 % (11/280, 95 % CI 2.1–6.9) of all male specimens, in 7.7 % (10/130, 95 % CI 4.1–13.7) of patients with non-gonococcal urethritis (NGU) and in 0.7 % (1/150, 95 % CI &lt;0.01–4.1) of patients without urethritis. The presence of <jats:italic>M. genitalium</jats:italic> was significantly associated with NGU (<jats:italic>P</jats:italic> ≤0.01; 95 % Cl 0.88–0.98) and non-chlamydial-non-gonococcal urethritis (<jats:italic>P</jats:italic>=0.0005; 95 % Cl 0.84–0.97).</jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19502372","pmcid":null,"openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.009977-0?crawler=true","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":["Humans","Mycoplasma genitalium","Mycoplasma Infections","Urethritis","Sensitivity and Specificity","Case-Control Studies","Double-Blind Method","Polymerase Chain Reaction","Gene Expression Regulation, Bacterial","Genes, Bacterial","Adult","Sexual Partners","Female","Male"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T04:01:48.968544Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}