{"doi":"10.1099/jgv.0.000410","title":"Localization of neutralization epitopes on adenovirus fiber knob from species C","abstract":null,"journal":"Journal of General Virology","year":2016,"id":637976,"datarank":0.29188652235829704,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.0,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1656793,"name":"Lizheng Wang","orcid":null,"position":1,"is_corresponding":false},{"id":810407,"name":"Zixuan Wang","orcid":"0000-0003-1421-2824","position":2,"is_corresponding":false},{"id":477500,"name":"Rui Zhu","orcid":"0000-0002-6543-7380","position":3,"is_corresponding":false},{"id":488158,"name":"Jingyi Yan","orcid":"0000-0003-2978-3769","position":4,"is_corresponding":false},{"id":1518341,"name":"Baoming Wang","orcid":null,"position":5,"is_corresponding":false},{"id":952944,"name":"Jiaxin Wu","orcid":"0000-0003-0618-2469","position":6,"is_corresponding":false},{"id":1118358,"name":"Haihong Zhang","orcid":"0000-0003-3765-1942","position":7,"is_corresponding":false},{"id":81081,"name":"Hui Wu","orcid":"0000-0002-7649-4575","position":8,"is_corresponding":false},{"id":436613,"name":"Yan Zhou","orcid":"0000-0002-5545-5681","position":9,"is_corresponding":false},{"id":212867,"name":"Wei Kong","orcid":null,"position":10,"is_corresponding":false},{"id":634346,"name":"Bin Yu","orcid":"0000-0002-3109-2062","position":11,"is_corresponding":false},{"id":530209,"name":"Xianghui Yu","orcid":"0000-0001-7739-1386","position":12,"is_corresponding":false},{"id":1656791,"name":"Shuai Lang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Localization of neutralization epitopes on adenovirus fiber knob from species C","abstract":"Although potential neutralization epitopes on the fiber knob of adenovirus (AdV) serotype 2 (Ad2) and Ad5 have been revealed, few studies have been carried out to identify neutralization epitopes on the knob from a broader panel of AdV serotypes. In this study, based on sequence and structural analysis of knobs from Ad1, Ad2, Ad5 and Ad6 (all from species C), several trimeric chimeric knob proteins were expressed in Escherichia coli to identify the locations of neutralization epitopes on the knobs by analysing their reactivity with mouse and rabbit polyclonal sera raised against AdVs and human sera with natural AdV infection. The dominant neutralization epitopes were located mainly in the N-terminal part of knobs from Ad1, Ad2 and Ad5, but they seemed to be located in the C-terminal part of the Ad6 knob, with some individual differences in rabbit and human populations. Our study adds to our understanding of humoral immune responses to AdVs and will facilitate the construction of more desirable capsid-modified recombinant Ad5 vectors.","is_dataset_classified":null,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26801881","pmcid":null,"openalex_id":"https://openalex.org/W2302386893","authors":[],"funders":[],"total_grants":0,"fwci":0.8679,"citation_percentile":0.79973544,"influential_citations":0,"citation_trend":[{"year":2018,"count":3},{"year":2022,"count":1},{"year":2024,"count":2}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000410?crawler=true","host_type":"publisher"},{"url":"https://doi.org/10.1099/jgv.0.000410","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/26801881","host_type":"repository"}],"fields_of_study":["Virus-based gene therapy research","Viral Infectious Diseases and Gene Expression in Insects","CAR-T cell therapy research","Adenoviridae","Adenoviridae Infections","Amino Acid Sequence","Animals","Cloning, Molecular","Epitope Mapping","Epitopes","Escherichia coli","Gene Expression","Green Fluorescent Proteins","HEK293 Cells","Humans","Immune Sera","Mice","Molecular Sequence Data","Neutralization Tests","Plasmids","Rabbits","Recombinant Proteins","Sequence Alignment","Serogroup"],"mesh_terms":["Adenoviridae","Adenoviridae Infections","Amino Acid Sequence","Animals","Epitopes","Cloning, Molecular","Escherichia coli","Humans","Immune Sera","Molecular Sequence Data","Neutralization Tests","Plasmids","Rabbits","Recombinant Proteins","Gene Expression","Sequence Alignment","Epitope Mapping","Green Fluorescent Proteins","Mice","HEK293 Cells","Serogroup"],"keywords":["Neutralization","Epitope","Polyclonal antibodies","Virology","Capsid","Biology","Serotype","Epitope mapping","Recombinant DNA","Mastadenovirus","Adenoviridae","Antibody","Gene","Virus","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T19:50:15.839538Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}