{"doi":"10.1099/ijsem.0.006516","title":"Characterization of gut symbionts from wild-caught Drosophila and other Diptera: description of Utexia brackfieldae gen. nov., sp. nov., Orbus sturtevantii sp. nov., Orbus wheelerorum sp. nov, and Orbus mooreae sp. nov.","abstract":"Non-culture based surveys show that the bacterial family Orbaceae is widespread in guts of insects, including wild Drosophila . Relatively few isolates have been described, and none has been described from Drosophila . We present the isolation and characterization of five strains of Orbaceae from wild-caught flies of the genera Drosophila (Diptera: Drosophilidae) and Neogriphoneura (Diptera: Lauxaniidae). Cells are generally rod-shaped, mesophilic, and measure 0.8–2.0 µm long by 0.3–0.5 µm wide. Optimal growth was observed under ambient atmosphere. Reconstruction of phylogenies from the 16S rRNA gene and from single-copy orthologs verify placement of these strains within Orbaceae . Cells exhibited similar fatty acid profiles to those of other Orbaceae . Strain lpD01 T shared 74% average nucleotide identity (ANI) with its closest relatives Ca. Schmidhempelia bombi Bimp and Zophobihabitans entericus IPMB12 T . Results from multiple genome-wide similarity comparisons indicate lpD01 T should be classified as a novel species within a novel genus. The major respiratory quinone for lpD01 T is ubiquinone Q-8. lpD02 T , lpD03, lpD04 T , and BiB T are more closely related to Orbus hercynius CN3 T (76, 77, 76, and 77% ANI, respectively) than to other described Orbaceae . Genomic and phylogenetic analyses suggest that lpD03 and lpD04 T belong to the same species and that lpD02 T , lpD03/lpD04 T , and BiB T are each novel species of the genus Orbus . The proposed names of these strains are Utexia brackfieldae gen. nov., sp. nov. (type strain lpD01 T =NCIMB 15517 T =ATCC TSD-399 T ), Orbus sturtevantii sp. nov (type strain lpD02 T =NCIMB 15518 T =ATCC TSD-400 T ), Orbus wheelerorum sp. nov. (type strain lpD04 T =NCIMB 15520 T =ATCC TSD-401 T ), and Orbus mooreae sp. nov (type strain BiB T =NCIMB 15516 T =ATCC TSD-402 T ). The isolation and characterization of these strains expands the repertoire of culturable bacteria naturally associated with insects, including the model organism D. melanogaster .","journal":"INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY","year":2024,"id":445604,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9429,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1262445,"name":"Kathleen Sotelo","orcid":null,"position":1,"is_corresponding":false},{"id":260626,"name":"Nancy A. Moran","orcid":"0000-0003-2983-9769","position":2,"is_corresponding":false},{"id":530266,"name":"Laila Phillips","orcid":"0000-0002-9565-0246","position":0,"is_corresponding":true}],"reference_count":75,"raw_metadata":null,"created_at":"2026-07-19T02:01:46.243344Z","pmid":"39331838","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}