{"doi":"10.1098/rsob.160019","title":"Cross-regulation between Aurora B and Citron kinase controls midbody architecture in cytokinesis","abstract":"<jats:p>Cytokinesis culminates in the final separation, or abscission, of the two daughter cells at the end of cell division. Abscission relies on an organelle, the midbody, which forms at the intercellular bridge and is composed of various proteins arranged in a precise stereotypic pattern. The molecular mechanisms controlling midbody organization and function, however, are obscure. Here we show that proper midbody architecture requires cross-regulation between two cell division kinases, Citron kinase (CIT-K) and Aurora B, the kinase component of the chromosomal passenger complex (CPC). CIT-K interacts directly with three CPC components and is required for proper midbody architecture and the orderly arrangement of midbody proteins, including the CPC. In addition, we show that CIT-K promotes Aurora B activity through phosphorylation of the INCENP CPC subunit at the TSS motif. In turn, Aurora B controls CIT-K localization and association with its central spindle partners through phosphorylation of CIT-K's coiled coil domain. Our results identify, for the first time, a cross-regulatory mechanism between two kinases during cytokinesis, which is crucial for establishing the stereotyped organization of midbody proteins.</jats:p>","journal":"Open Biology","year":2016,"id":42033,"datarank":1.7889410613725671,"base_score":3.970291913552122,"endowment":3.970291913552122,"self_citation_contribution":0.5955437870328184,"citation_network_contribution":1.1933972743397487,"self_endowment_contribution":0.5955437870328184,"citer_contribution":1.1933972743397487,"corpus_percentile":null,"corpus_rank":null,"citation_count":52,"citer_count":39,"citers_with_citation_signal":35,"citers_with_endowment":35,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":67.5,"fair_percentile":96.41600703605981,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":201708,"name":"Zuni I. Bassi","orcid":"0000-0003-4309-9656","position":1,"is_corresponding":false},{"id":201709,"name":"Janusz Debski","orcid":null,"position":2,"is_corresponding":false},{"id":201710,"name":"Marco Gottardo","orcid":null,"position":3,"is_corresponding":false},{"id":201711,"name":"Giuliano Callaini","orcid":null,"position":4,"is_corresponding":false},{"id":201712,"name":"Michal Dadlez","orcid":null,"position":5,"is_corresponding":false},{"id":201713,"name":"Pier Paolo D'Avino","orcid":null,"position":6,"is_corresponding":false},{"id":201707,"name":"Callum McKenzie","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.970291913552122,"endowment":3.970291913552122,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27009191","pmcid":"PMC4821246","openalex_id":"https://openalex.org/W2309715007","authors":[],"funders":[],"total_grants":0,"fwci":3.4045,"citation_percentile":0.91608368,"influential_citations":2,"citation_trend":[{"year":2016,"count":4},{"year":2017,"count":2},{"year":2018,"count":3},{"year":2019,"count":9},{"year":2020,"count":15},{"year":2021,"count":5},{"year":2022,"count":2},{"year":2023,"count":2},{"year":2024,"count":2},{"year":2025,"count":5},{"year":2026,"count":3}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.1098/rsob.160019","host_type":"journal"},{"url":"https://doi.org/10.1098/rsob.160019","host_type":"GOLD"},{"url":"https://doi.org/10.1098/rsob.160019","host_type":"publisher"},{"url":"https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.160019","host_type":"publisher"},{"url":"https://royalsocietypublishing.org/doi/full-xml/10.1098/rsob.160019","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27009191","host_type":"repository"},{"url":"https://doaj.org/article/883751350a634409a90388e72e89edd9","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4821246","host_type":"repository"},{"url":"http://hdl.handle.net/11365/1006581","host_type":"repository"},{"url":"https://www.repository.cam.ac.uk/handle/1810/254044","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4821246","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4821246?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Microtubule and mitosis dynamics","Cellular transport and secretion","Protist diversity and phylogeny","Biology","Medicine","Aurora Kinase B","Cell Division","Cytokinesis","HeLa Cells","Humans","Intracellular Signaling Peptides and Proteins","Phosphorylation","Protein Interaction Maps","Protein Serine-Threonine Kinases","Spindle Apparatus"],"mesh_terms":["Cell Division","HeLa Cells","Humans","Spindle Apparatus","Phosphorylation","Protein Serine-Threonine Kinases","Intracellular Signaling Peptides and Proteins","Cytokinesis","Protein Interaction Maps","Aurora Kinase B","Hela Cells"],"keywords":["Midbody","Cytokinesis","Aurora B kinase","Cell biology","Biology","Abscission","Aurora inhibitor","Kinase","Cell division","Organelle","Genetics","Cell","Cell cycle","Aurora B","Citron Kinase"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"refseq"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-13T18:54:05.191292Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":100.0,"fair_a":70.0,"fair_i":50.0,"fair_r":50.0,"fair_zscore":2.0161,"fair_rationale":{"fair_score":67.5,"has_llm":false,"dimensions":{"F":{"name":"Findable","score":100.0,"criteria":[{"key":"f_has_doi","label":"Has a persistent DOI","kind":"deterministic","weight":1.0,"fraction":1.0,"signal":"DOI present","rationale":null},{"key":"f_repository_presence","label":"Indexed in repositories / literature DBs","kind":"deterministic","weight":1.0,"fraction":1.0,"signal":"datacite=0, pmcid=True, pmid=True","rationale":null},{"key":"f_persistent_ids","label":"Resolvable scholarly identifiers (OpenAlex)","kind":"deterministic","weight":0.5,"fraction":1.0,"signal":"OpenAlex id present","rationale":null}]},"A":{"name":"Accessible","score":70.0,"criteria":[{"key":"a_open_access","label":"Open Access / files deposited","kind":"deterministic","weight":1.5,"fraction":0.5,"signal":"files/OA location present but not flagged OA","rationale":null},{"key":"a_retrievable","label":"Free full text retrievable","kind":"deterministic","weight":1.0,"fraction":1.0,"signal":"12 OA location(s)","rationale":null}]},"I":{"name":"Interoperable","score":50.0,"criteria":[{"key":"i_linked_data","label":"Linked datasets / DataCite relations","kind":"deterministic","weight":1.0,"fraction":0.0,"signal":"linked_datasets=0, datacite=0","rationale":null},{"key":"i_standard_ids","label":"References data via standard accessions","kind":"deterministic","weight":1.0,"fraction":1.0,"signal":"accessions=1, trials=0","rationale":null}]},"R":{"name":"Reusable","score":50.0,"criteria":[{"key":"r_license","label":"Clear, open reuse license","kind":"deterministic","weight":1.5,"fraction":1.0,"signal":"open license (cc-by)","rationale":null},{"key":"r_downloads","label":"Demonstrated reuse (downloads)","kind":"deterministic","weight":0.5,"fraction":0.0,"signal":"downloads=0","rationale":null},{"key":"r_version","label":"Versioned / maintained","kind":"deterministic","weight":0.5,"fraction":0.0,"signal":"no version chain","rationale":null},{"key":"r_dataset","label":"Classified as a data resource","kind":"deterministic","weight":0.5,"fraction":0.0,"signal":"not a dataset","rationale":null}]}},"suggestions":["Link the underlying datasets via DOIs / DataCite relations.","Maintain explicit versioning for the dataset.","Make the paper/data Open Access or deposit the files in an open repository."],"model":null,"agent_version":"fair_agent_v1","fulltext_source":"epmc_xml"},"fair_model":null,"fair_agent_version":"fair_agent_v1","fair_fulltext_source":"epmc_xml","fair_has_llm":false,"fair_computed_at":"2026-06-13T20:49:49.887188Z","clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}