{"doi":"10.1098/rsif.2010.0445","title":"Electroporation and lysis of marine microalga\n                    <i>Karenia brevis</i>\n                    for RNA extraction and amplification","abstract":"<jats:p>\n                    We describe here a simple device for dielectrophoretic concentration of marine microalga\n                    <jats:italic>Karenia brevis</jats:italic>\n                    non-motile cells, followed by electric field-mediated lysis for RNA extraction. The lysate was purified using magnetic beads and pure RNA extracted. RNA quality was assessed off-chip by nucleic acid sequence-based amplification and the optimum conditions for lysis were determined. This procedure will form part of an integrated microfluidic system that is being developed with sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time quantitative RNA detection. The integrated system and its components could be used for a large range of applications including\n                    <jats:italic>in situ</jats:italic>\n                    harmful algal bloom detection, transcriptomics and point-of-care diagnostics.\n                  </jats:p>","journal":"Journal of The Royal Society Interface","year":2011,"id":673676,"datarank":0.5375278407684165,"base_score":3.58351893845611,"endowment":3.58351893845611,"self_citation_contribution":0.5375278407684165,"citation_network_contribution":0.0,"self_endowment_contribution":0.5375278407684165,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":35,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1760113,"name":"M.-N. 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RNA quality was assessed off-chip by nucleic acid sequence-based amplification and the optimum conditions for lysis were determined. This procedure will form part of an integrated microfluidic system that is being developed with sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time quantitative RNA detection. The integrated system and its components could be used for a large range of applications including\n                    <jats:italic>in situ</jats:italic>\n                    harmful algal bloom detection, transcriptomics and point-of-care diagnostics.\n                  </jats:p>","is_dataset_classified":null,"base_score":3.58351893845611,"endowment":3.58351893845611,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21084344","pmcid":"PMC3061125","openalex_id":"https://openalex.org/W2159120652","authors":[],"funders":[{"funder_name":"Natural Environment Research Council","grant_id":"noc010003","title":null},{"funder_name":"European Commission FP7","grant_id":"FP7_224306","title":null},{"funder_name":"Natural Environment Research Council","grant_id":"noc010013","title":null},{"funder_name":"European Commission","grant_id":"224306","title":"Laboratory Skin Patches and SmartCards based on foils and compatible with a smartphone"}],"total_grants":4,"fwci":1.2895,"citation_percentile":0.7993577,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":4},{"year":2014,"count":2},{"year":2015,"count":2},{"year":2016,"count":3},{"year":2017,"count":2},{"year":2018,"count":2},{"year":2019,"count":2},{"year":2020,"count":2},{"year":2021,"count":4},{"year":2022,"count":1},{"year":2023,"count":3},{"year":2024,"count":1},{"year":2025,"count":3},{"year":2026,"count":1}],"oa_status":"green","license":"Royal Society Data Sharing and Accessibility","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3061125","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3061125","host_type":"repository"},{"url":"https://royalsocietypublishing.org/doi/pdf/10.1098/rsif.2010.0445","host_type":"publisher"},{"url":"https://royalsocietypublishing.org/doi/full-xml/10.1098/rsif.2010.0445","host_type":"publisher"},{"url":"https://doi.org/10.1098/rsif.2010.0445","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/21084344","host_type":"repository"},{"url":"https://eprints.soton.ac.uk/171055/","host_type":"repository"},{"url":"http://nora.nerc.ac.uk/id/eprint/271055/","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc3061125?pdf=render","host_type":""},{"url":"https://dx.doi.org/10.1098/rsif.2010.0445","host_type":""},{"url":"https://doi.org/https://doi.org/10.1098/rsif.2010.0445","host_type":""}],"fields_of_study":["Microfluidic and Bio-sensing Technologies","Electrohydrodynamics and Fluid Dynamics","Magnetic and Electromagnetic Effects","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["RNA","Electroporation","Cell Culture Techniques","Nucleic Acid Amplification Techniques","Microfluidics","Alveolata","Microalgae"],"keywords":["Lysis","RNA","RNA extraction","Nucleic acid","Electroporation","Lysis buffer","Biology","Extraction (chemistry)","Alkaline lysis","Chromatography","DNA","Chemistry","Molecular biology","Biochemistry","Gene","Plasmid","570","Alveolata","Microfluidics","Cell Culture Techniques","Microalgae","Nucleic Acid Amplification Techniques"],"sdg_mappings":[{"sdg_number":6,"sdg_label":"6. 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