{"doi":"10.1097/mbc.0b013e32835496b2","title":"TA-2, a thrombin-like enzyme from the Chinese white-lipped green pitviper (Trimeresurus albolabris)","abstract":"Through three chromatographic steps, a new thrombin-like enzyme (TLE), named TA-2, from the venom of the Chinese white-lipped green pitviper (Trimeresurus albolabris) has been isolated and purified to homogeneity. TA-2 was a single-chain glycoprotein with about 6% sugar, pI 3.9 and a molecular weight of 38.8 kD. Its N-terminal sequence (VVGGDECNIN) showed high sequence conformity with many other TLEs. In vitro, it coagulated bovine fibrinogen (108.6 NIH units/mg) and cleaved the Aα and Bβ chains of bovine fibrinogen-releasing fibrinopeptide A and B, but did not degrade bovine fibrin; displayed high stability at different temperature, pH, and presence of several divalent cations and inhibitors; also exhibited strong activity towards casein (192.3 units/mg) and high esterase activity upon Nα-p-tosyl-L-arginine methyl ester (11 units/mg); and behaved as a promoter to platelet aggregation induced by ADP or collagen. In vivo, TA-2 caused dose-dependent prolongation of bleeding time in mice, but had no hemorrhagic and edema-inducing activities even at high concentrations.","journal":"Blood Coagulation & Fibrinolysis","year":2012,"id":3257,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.0442,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2012-07-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":30887,"name":"Alexandra P. Lewis","orcid":"0000-0002-6195-4786","position":1,"is_corresponding":false},{"id":34958,"name":"Bin Zhou","orcid":"0000-0003-4188-4378","position":2,"is_corresponding":false},{"id":34959,"name":"Haoping Wen","orcid":null,"position":3,"is_corresponding":false},{"id":34960,"name":"Jingbo Li","orcid":"0000-0001-5960-806X","position":4,"is_corresponding":false},{"id":34961,"name":"Bin Xiao","orcid":"0000-0001-6813-3406","position":5,"is_corresponding":false},{"id":2996,"name":"Kang Zhang","orcid":"0000-0002-4549-1697","position":6,"is_corresponding":false},{"id":34962,"name":"Wayne C. Hodgson","orcid":"0000-0002-9919-0144","position":7,"is_corresponding":false},{"id":34963,"name":"Xiaodong Yu","orcid":null,"position":8,"is_corresponding":false},{"id":34957,"name":"Qiyi He","orcid":"0000-0003-3567-5772","position":0,"is_corresponding":true}],"reference_count":45,"raw_metadata":null,"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}