{"doi":"10.1096/fj.202002610r","title":"Exomer complex regulates protein traffic at the TGN through differential interactions with cargos and clathrin adaptor complexes","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>\n                    Protein sorting at the\n                    <jats:italic>trans</jats:italic>\n                    ‐Golgi network (TGN) usually requires the assistance of cargo adaptors. However, it remains to be examined how the same complex can mediate both the export and retention of different proteins or how sorting complexes interact among themselves. In\n                    <jats:italic>Saccharomyces cerevisiae</jats:italic>\n                    , the exomer complex is involved in the polarized transport of some proteins from the TGN to the plasma membrane (PM). Intriguingly, exomer and its cargos also show a sort of functional relationship with TGN clathrin adaptors that is still unsolved. Here, using a wide range of techniques, including time‐lapse and BIFC microscopy, we describe new molecular implications of the exomer complex in protein sorting and address its different layers of functional interaction with clathrin adaptor complexes. Exomer mutants show impaired amino acid uptake because it facilitates not only the polarized delivery of amino acid permeases to the PM but also participates in their endosomal traffic. We propose a model for exomer where it modulates the recruitment of TGN clathrin adaptors directly or indirectly through the Arf1 function. Moreover, we describe an in vivo competitive relationship between the exomer and AP‐1 complexes for the model cargo Chs3. These results highlight a broad role for exomer in regulating protein sorting at the TGN that is complementary to its role as cargo adaptor and present a model to understand the complexity of TGN protein sorting.\n                  </jats:p>","journal":"The FASEB Journal","year":2021,"id":24273,"datarank":0.5843438869401248,"base_score":2.833213344056216,"endowment":2.833213344056216,"self_citation_contribution":0.42498200160843247,"citation_network_contribution":0.15936188533169238,"self_endowment_contribution":0.42498200160843247,"citer_contribution":0.15936188533169238,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":11,"citers_with_citation_signal":9,"citers_with_endowment":9,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":146196,"name":"Noelia Sanchez","orcid":null,"position":1,"is_corresponding":false},{"id":146197,"name":"Rosario Valle","orcid":null,"position":2,"is_corresponding":false},{"id":146198,"name":"Jose Miguel Mulet","orcid":null,"position":3,"is_corresponding":false},{"id":146199,"name":"Mara C. Duncan","orcid":null,"position":4,"is_corresponding":false},{"id":146200,"name":"Cesar Roncero","orcid":null,"position":5,"is_corresponding":false},{"id":146195,"name":"Carlos Anton‐Plagaro","orcid":"0000-0002-7497-0080","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.833213344056216,"endowment":2.833213344056216,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"33978245","pmcid":"PMC9066374","openalex_id":"https://openalex.org/W3162365722","authors":[],"funders":[{"funder_name":"Ministerio de Economía, Industria y Competitividad, Gobierno de España","grant_id":"CICYT/FEDER BFU2017‐84508‐P","title":null},{"funder_name":"Consejería de Educación, Junta de Castilla y León","grant_id":"SA116G19","title":null},{"funder_name":"Ministerio de Economía, Industria y Competitividad, Gobierno de España","grant_id":"RTC‐2017‐6468‐2‐AR","title":null},{"funder_name":"Ministerio de Economía, Industria y Competitividad, Gobierno de España","grant_id":"BIO2016‐77776‐P","title":null},{"funder_name":"Foundation for the National Institutes of Health","grant_id":"R01 GM092741","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01GM092741-06","title":"Regulatory mechanisms of clathrin dependant traffic at the TGN and endosomes"}],"total_grants":6,"fwci":1.2964,"citation_percentile":0.76969345,"influential_citations":0,"citation_trend":[{"year":2021,"count":2},{"year":2022,"count":3},{"year":2023,"count":5},{"year":2024,"count":2},{"year":2025,"count":3},{"year":2026,"count":1}],"oa_status":"bronze","license":"CC BY NC","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1096/fj.202002610R","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1096/fj.202002610R","host_type":"HYBRID"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1096/fj.202002610R","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1096/fj.202002610R","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full-xml/10.1096/fj.202002610R","host_type":"publisher"},{"url":"https://faseb.onlinelibrary.wiley.com/doi/pdf/10.1096/fj.202002610R","host_type":"publisher"},{"url":"https://doi.org/10.1096/fj.202002610r","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/33978245","host_type":"repository"},{"url":"https://hdl.handle.net/2027.42/167830","host_type":"repository"},{"url":"http://hdl.handle.net/10261/261255","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9066374","host_type":"repository"},{"url":"http://hdl.handle.net/10251/189344","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC9066374","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC9066374?pdf=render","host_type":"Europe_PMC"},{"url":"https://riunet.upv.es/handle/10251/189344","host_type":""},{"url":"https://dx.doi.org/10.1096/fj.202002610R","host_type":""},{"url":"https://dx.doi.org/10.1096/fj.202002610r","host_type":""},{"url":"https://doi.org/https://doi.org/10.1096/fj.202002610R","host_type":""}],"fields_of_study":["Cellular transport and secretion","Endoplasmic Reticulum Stress and Disease","RNA regulation and disease","Medicine","Biology","0301 basic medicine","03 medical and health sciences","0303 health sciences","ADP-Ribosylation Factor 1","Adaptor Proteins, Vesicular Transport","Cell Membrane","Chitin Synthase","Endosomes","Protein Transport","Saccharomyces cerevisiae","Saccharomyces cerevisiae Proteins","trans-Golgi Network"],"mesh_terms":["Cell Membrane","Chitin Synthase","Endosomes","Saccharomyces cerevisiae","ADP-Ribosylation Factor 1","Protein Transport","trans-Golgi Network","Saccharomyces cerevisiae Proteins","Adaptor Proteins, Vesicular Transport"],"keywords":["Signal transducing adaptor protein","Clathrin","Clathrin adaptor proteins","Cell biology","Retromer","Endosome","Protein targeting","Golgi apparatus","Transport protein","Sorting","Chemistry","Biology","Membrane protein","Membrane","Biochemistry","Vesicle","Signal transduction","Computer science","Endosomes","Tgn","Clathrin Adaptor","Exomer","Intracelullar Traffic","Clathrin adaptors","Saccharomyces cerevisiae Proteins","Science","Saccharomyces cerevisiae","15.- Proteger, restaurar y promover la utilización sostenible de los ecosistemas terrestres, gestionar de manera sostenible los bosques, combatir la desertificación y detener y revertir la degradación de la tierra, y frenar la pérdida de diversidad biológica","03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades","Traffic","Chitin Synthase","Cell Membrane","08.- Fomentar el crecimiento económico sostenido, inclusivo y sostenible, el empleo pleno y productivo, y el trabajo decente para todos","Adaptor Proteins, Vesicular Transport","Protein Transport","ADP-Ribosylation Factor 1","Intracelullar","trans-Golgi 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