{"doi":"10.1093/trstmh/traa148","title":"Drug-resistant <i>Mycobacterium tuberculosis</i> and its genotypes isolated from an outbreak in western Thailand","abstract":"BACKGROUND: Multidrug-resistant TB (MDR-TB) outbreaks have occurred in the Thamaka district, Kanchanaburi province in Thailand. METHODS: Seventy-two isolates, which included 7% mono-, 30.6% MDR and extensively drug-resistant TB (XDR-TB), were genotyped by spoligotyping, mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) and single nucleotide polymorphism genotyping, and their drug resistance was analysed. RESULTS: The spoligotyping results showed that Beijing spoligo-international type (SIT)1 was predominant (n=38; 52.8%) while the remaining were non-Beijing sublineages (n=34). The MIRU-VNTR analysis showed that Beijing isolates, most of which belonged to the modern type (n=37), formed 5 clusters and 13 individual patterns. In katG, only mutation Ser315Thr was identified. In rpoB, Ser531Leu was predominant, except for His526Arg and Leu533Pro, which were found in two isolates. A cluster of 14 Beijing strains contained these common mutations and shared the MIRU-VNTR genotype with isolates in the Thamaka district that had spread previously. Two U SIT523 isolates contained the mutations A1400G in rrs and Asp94Gly in gyrA genes, indicating a spread of XDR-TB. CONCLUSIONS: Most mutations were associated with drug resistance and the specific MDR Beijing and XDR-TB in U SIT523 isolates remain. This genotyping is a key tool for tracking TB transmission in the Thamaka district of Thailand.","journal":"Transactions of the Royal Society of Tropical Medicine and Hygiene","year":2020,"id":85103,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8932,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":435810,"name":"Benjawan Phetsuksiri","orcid":"0000-0001-6507-2898","position":1,"is_corresponding":false},{"id":435811,"name":"Chie Nakajima","orcid":"0000-0002-9194-1062","position":2,"is_corresponding":false},{"id":436850,"name":"Supranee Bunchoo","orcid":null,"position":3,"is_corresponding":false},{"id":436851,"name":"Krairerk Suthum","orcid":null,"position":4,"is_corresponding":false},{"id":436852,"name":"Nattakan Tipkrua","orcid":null,"position":5,"is_corresponding":false},{"id":435812,"name":"Yukari Fukushima","orcid":"0000-0001-5273-4135","position":6,"is_corresponding":false},{"id":435813,"name":"Yasuhiko Suzuki","orcid":"0000-0002-1313-1494","position":7,"is_corresponding":false},{"id":435809,"name":"Janisara Rudeeaneksin","orcid":"0009-0001-4859-7243","position":0,"is_corresponding":true}],"reference_count":36,"raw_metadata":null,"created_at":"2026-07-18T21:56:02.794896Z","pmid":"33320938","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}