{"doi":"10.1093/procel/pwae025","title":"NudCL2 is required for cytokinesis by stabilizing RCC2 with Hsp90 at the midbody","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>Cytokinesis is required for faithful division of cytoplasmic components and duplicated nuclei into two daughter cells. Midbody, a protein-dense organelle that forms at the intercellular bridge, is indispensable for successful cytokinesis. However, the regulatory mechanism of cytokinesis at the midbody still remains elusive. Here, we unveil a critical role for NudC-like protein 2 (NudCL2), a co-chaperone of heat shock protein 90 (Hsp90), in cytokinesis regulation by stabilizing regulator of chromosome condensation 2 (RCC2) at the midbody in mammalian cells. NudCL2 localizes at the midbody, and its downregulation results in cytokinesis failure, multinucleation, and midbody disorganization. Using iTRAQ-based quantitative proteomic analysis, we find that RCC2 levels are decreased in NudCL2 knockout (KO) cells. Moreover, Hsp90 forms a complex with NudCL2 to stabilize RCC2, which is essential for cytokinesis. RCC2 depletion mirrors phenotypes observed in NudCL2-downregulated cells. Importantly, ectopic expression of RCC2 rescues the cytokinesis defects induced by NudCL2 deletion, but not vice versa. Together, our data reveal the significance of the NudCL2/Hsp90/RCC2 pathway in cytokinesis at the midbody.</jats:p>","journal":"Protein &amp; Cell","year":2024,"id":598517,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":518922,"name":"Yuliang Huang","orcid":"0000-0001-9994-472X","position":1,"is_corresponding":false},{"id":60388,"name":"Feng Yang","orcid":"0000-0003-0769-5573","position":2,"is_corresponding":false},{"id":201515,"name":"Xiaoxia Sun","orcid":null,"position":3,"is_corresponding":false},{"id":201516,"name":"Rijin Lin","orcid":null,"position":4,"is_corresponding":false},{"id":991870,"name":"Jiaxing Feng","orcid":"0000-0003-4493-3534","position":5,"is_corresponding":false},{"id":201518,"name":"Mingyang Yang","orcid":null,"position":6,"is_corresponding":false},{"id":201519,"name":"Jiaqi Shao","orcid":null,"position":7,"is_corresponding":false},{"id":204412,"name":"Xiaoqi Liu","orcid":null,"position":8,"is_corresponding":false},{"id":201520,"name":"Tianhua Zhou","orcid":"0000-0002-1791-2124","position":9,"is_corresponding":false},{"id":1533708,"name":"Shanshan Xie","orcid":"0000-0003-4294-8169","position":10,"is_corresponding":false},{"id":201521,"name":"Yuehong Yang","orcid":"0000-0001-5438-8395","position":11,"is_corresponding":false},{"id":494375,"name":"Xiaoyang Xu","orcid":"0000-0002-1634-3329","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"NudCL2 is required for cytokinesis by stabilizing RCC2 with Hsp90 at the midbody","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>Cytokinesis is required for faithful division of cytoplasmic components and duplicated nuclei into two daughter cells. Midbody, a protein-dense organelle that forms at the intercellular bridge, is indispensable for successful cytokinesis. However, the regulatory mechanism of cytokinesis at the midbody still remains elusive. Here, we unveil a critical role for NudC-like protein 2 (NudCL2), a co-chaperone of heat shock protein 90 (Hsp90), in cytokinesis regulation by stabilizing regulator of chromosome condensation 2 (RCC2) at the midbody in mammalian cells. NudCL2 localizes at the midbody, and its downregulation results in cytokinesis failure, multinucleation, and midbody disorganization. Using iTRAQ-based quantitative proteomic analysis, we find that RCC2 levels are decreased in NudCL2 knockout (KO) cells. Moreover, Hsp90 forms a complex with NudCL2 to stabilize RCC2, which is essential for cytokinesis. RCC2 depletion mirrors phenotypes observed in NudCL2-downregulated cells. Importantly, ectopic expression of RCC2 rescues the cytokinesis defects induced by NudCL2 deletion, but not vice versa. Together, our data reveal the significance of the NudCL2/Hsp90/RCC2 pathway in cytokinesis at the midbody.</jats:p>","is_dataset_classified":null,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"38801297","pmcid":"PMC11443449","openalex_id":"https://openalex.org/W4399063694","authors":[],"funders":[{"funder_name":"National Natural Science Foundation of China","grant_id":"32070709","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"32270771","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"U21A20197","title":null},{"funder_name":"National Key Research and Development Program of China","grant_id":"2019YFA0802202","title":null}],"total_grants":4,"fwci":0.4904,"citation_percentile":0.60357262,"influential_citations":0,"citation_trend":[{"year":2025,"count":3}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://academic.oup.com/proteincell/advance-article-pdf/doi/10.1093/procel/pwae025/57926879/pwae025.pdf","host_type":"journal"},{"url":"https://academic.oup.com/proteincell/advance-article-pdf/doi/10.1093/procel/pwae025/57926879/pwae025.pdf","host_type":"publisher"},{"url":"https://academic.oup.com/proteincell/advance-article-pdf/doi/10.1093/procel/pwae025/58147036/pwae025.pdf","host_type":"publisher"},{"url":"https://academic.oup.com/proteincell/article-pdf/15/10/766/59472689/pwae025.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/procel/pwae025","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/38801297","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11443449","host_type":"repository"},{"url":"https://uknowledge.uky.edu/markey_facpub/268","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC11443449","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC11443449?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Heat shock proteins research","Genetics, Aging, and Longevity in Model Organisms","Mitochondrial Function and Pathology","Cytokinesis","HSP90 Heat-Shock Proteins","Humans","HeLa Cells","Guanine Nucleotide Exchange Factors","Cell Cycle Proteins","Chromosomal Proteins, Non-Histone"],"mesh_terms":["Chromosomal Proteins, Non-Histone","HeLa Cells","Humans","Cell Cycle Proteins","HSP90 Heat-Shock Proteins","Guanine Nucleotide Exchange Factors","Cytokinesis","Hela Cells"],"keywords":["Midbody","Cytokinesis","Septin","Cell biology","Biology","Cell division","Genetics","Cell","HSP90","Rcc2","Nudcl2"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T15:50:18.787299Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}