{"doi":"10.1093/pnasnexus/pgac037","title":"Engineering a self-eliminating transgene in the yellow fever mosquito,<i>Aedes aegypti</i>","abstract":"Abstract Promising genetics-based approaches are being developed to reduce or prevent the transmission of mosquito-vectored diseases. Less clear is how such transgenes can be removed from the environment, a concern that is particularly relevant for highly invasive gene drive transgenes. Here, we lay the groundwork for a transgene removal system based on single-strand annealing (SSA), a eukaryotic DNA repair mechanism. An SSA-based rescuer strain (kmoRG) was engineered to have direct repeat sequences (DRs) in the Aedes aegypti kynurenine 3-monooxygenase (kmo) gene flanking the intervening transgenic cargo genes, DsRED and EGFP. Targeted induction of DNA double-strand breaks (DSBs) in the DsRED transgene successfully triggered complete elimination of the entire cargo from the kmoRG strain, restoring the wild-type kmo gene, and thereby, normal eye pigmentation. Our work establishes the framework for strategies to remove transgene sequences during the evaluation and testing of modified strains for genetics-based mosquito control.","journal":"PNAS Nexus","year":2022,"id":259660,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9537,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":912778,"name":"Chanell Dawson","orcid":null,"position":1,"is_corresponding":false},{"id":912296,"name":"Collin Valentin","orcid":"0000-0002-0316-1219","position":2,"is_corresponding":false},{"id":912779,"name":"Bryan Contreras","orcid":null,"position":3,"is_corresponding":false},{"id":912297,"name":"Josef Zapletal","orcid":"0000-0002-5569-5239","position":4,"is_corresponding":false},{"id":740211,"name":"Kevin M. Myles","orcid":"0000-0002-9820-2502","position":5,"is_corresponding":false},{"id":291982,"name":"Zach N. Adelman","orcid":"0000-0001-5901-7171","position":6,"is_corresponding":false},{"id":896560,"name":"Keun Chae","orcid":null,"position":0,"is_corresponding":true}],"reference_count":68,"raw_metadata":null,"created_at":"2026-07-19T00:25:58.993402Z","pmid":"36713320","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}