{"doi":"10.1093/plphys/kiab020","title":"Metabolic control of histone demethylase activity involved in plant response to high temperature","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>Jumonji C (JmjC) domain proteins are histone lysine demethylases that require ferrous iron and alpha-ketoglutarate (or α-KG) as cofactors in the oxidative demethylation reaction. In plants, α-KG is produced by isocitrate dehydrogenases (ICDHs) in different metabolic pathways. It remains unclear whether fluctuation of α-KG levels affects JmjC demethylase activity and epigenetic regulation of plant gene expression. In this work, we studied the impact of loss of function of the cytosolic ICDH (cICDH) gene on the function of histone demethylases in Arabidopsis thaliana. Loss of cICDH resulted in increases of overall histone H3 lysine 4 trimethylation (H3K4me3) and enhanced mutation defects of the H3K4me3 demethylase gene JMJ14. Genetic analysis suggested that the cICDH mutation may affect the activity of other demethylases, including JMJ15 and JMJ18 that function redundantly with JMJ14 in the plant thermosensory response. Furthermore, we show that mutation of JMJ14 affected both the gene activation and repression programs of the plant thermosensory response and that JMJ14 and JMJ15 repressed a set of genes that are likely to play negative roles in the process. The results provide evidence that histone H3K4 demethylases are involved in the plant response to elevated ambient temperature.</jats:p>","journal":"Plant Physiology","year":2021,"id":605762,"datarank":0.5837730447165941,"base_score":3.8918202981106265,"endowment":3.8918202981106265,"self_citation_contribution":0.5837730447165941,"citation_network_contribution":0.0,"self_endowment_contribution":0.5837730447165941,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":48,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":942094,"name":"Yu Zheng","orcid":"0000-0003-1223-2901","position":1,"is_corresponding":false},{"id":1070995,"name":"Yue Lu","orcid":"0009-0004-5465-367X","position":2,"is_corresponding":false},{"id":1554779,"name":"Emmanuelle Issakidis-Bourguet","orcid":null,"position":3,"is_corresponding":false},{"id":1224960,"name":"Dao‐Xiu Zhou","orcid":"0000-0002-1540-0598","position":4,"is_corresponding":false},{"id":1554778,"name":"Xiaoyun Cui","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Metabolic control of histone demethylase activity involved in plant response to high temperature","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>Jumonji C (JmjC) domain proteins are histone lysine demethylases that require ferrous iron and alpha-ketoglutarate (or α-KG) as cofactors in the oxidative demethylation reaction. In plants, α-KG is produced by isocitrate dehydrogenases (ICDHs) in different metabolic pathways. It remains unclear whether fluctuation of α-KG levels affects JmjC demethylase activity and epigenetic regulation of plant gene expression. In this work, we studied the impact of loss of function of the cytosolic ICDH (cICDH) gene on the function of histone demethylases in Arabidopsis thaliana. Loss of cICDH resulted in increases of overall histone H3 lysine 4 trimethylation (H3K4me3) and enhanced mutation defects of the H3K4me3 demethylase gene JMJ14. Genetic analysis suggested that the cICDH mutation may affect the activity of other demethylases, including JMJ15 and JMJ18 that function redundantly with JMJ14 in the plant thermosensory response. Furthermore, we show that mutation of JMJ14 affected both the gene activation and repression programs of the plant thermosensory response and that JMJ14 and JMJ15 repressed a set of genes that are likely to play negative roles in the process. The results provide evidence that histone H3K4 demethylases are involved in the plant response to elevated ambient temperature.</jats:p>","is_dataset_classified":null,"base_score":3.8918202981106265,"endowment":3.8918202981106265,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"33793949","pmcid":null,"openalex_id":"https://openalex.org/W3128652098","authors":[],"funders":[{"funder_name":"French Agence Nationale de la Recherche project","grant_id":"ANR-19-CE12-0027","title":"Redox regulation of plant HDAC activity in response to climate change"}],"total_grants":1,"fwci":6.3505,"citation_percentile":0.96130406,"influential_citations":0,"citation_trend":[{"year":2021,"count":1},{"year":2022,"count":5},{"year":2023,"count":12},{"year":2024,"count":13},{"year":2025,"count":12},{"year":2026,"count":5}],"oa_status":"bronze","license":"OUP Standard Publication Reuse","oa_locations":[{"url":"https://academic.oup.com/plphys/article-pdf/185/4/1813/37324619/kiab020.pdf","host_type":"journal"},{"url":"https://academic.oup.com/plphys/article-pdf/185/4/1813/37324619/kiab020.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/plphys/advance-article-pdf/doi/10.1093/plphys/kiab020/36138796/kiab020.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/plphys/article-pdf/185/4/1813/37324619/kiab020.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/plphys/kiab020","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/33793949","host_type":"repository"},{"url":"https://hal.inrae.fr/hal-03331757","host_type":"repository"},{"url":"https://hal.archives-ouvertes.fr/hal-03409784","host_type":"repository"},{"url":"https://hal-cnrs.archives-ouvertes.fr/hal-03409785","host_type":""},{"url":"https://hal.science/hal-04452038","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8133595","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133595","host_type":""},{"url":"https://dx.doi.org/10.1093/plphys/kiab020","host_type":""},{"url":"https://hal.inrae.fr/hal-03331757v1","host_type":""},{"url":"https://doi.org/https://doi.org/10.1093/plphys/kiab020","host_type":""}],"fields_of_study":["Plant Molecular Biology Research","Light effects on plants","Photosynthetic Processes and Mechanisms","0301 basic medicine","03 medical and health sciences","Arabidopsis","Arabidopsis Proteins","Gene Expression Regulation, Plant","Genetic Variation","Genotype","Histone Demethylases","Hot Temperature","Mutation","Plants, Genetically Modified","Stress, Physiological"],"mesh_terms":["Genotype","Hot Temperature","Mutation","Stress, Physiological","Genetic Variation","Arabidopsis","Gene Expression Regulation, Plant","Arabidopsis Proteins","Plants, Genetically Modified","Histone Demethylases"],"keywords":["Demethylase","Histone","Biology","Chemistry","Biochemistry","Cell biology","Gene","570","Hot Temperature","Genotype","[SDV]Life Sciences [q-bio]","epigenetic Control","Arabidopsis","610","perception","Gene Expression Regulation, Plant","Stress, Physiological","Dependent Isocitrate Dehydrogenase","[SDV.BV]Life Sciences [q-bio]/Vegetal Biology","[SDV.BV] Life Sciences [q-bio]/Vegetal Biology","transcription Factor","580","Histone Demethylases","drought Stress","Arabidopsis Proteins","h3 Lysine 4","Genetic Variation","Plants, Genetically Modified","inhibition","jmj14","Mutation","flowering Time"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T03:11:12.857931Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}