{"doi":"10.1093/pcmedi/pbae007","title":"Genome and clonal hematopoiesis stability contrasts with immune, cfDNA, mitochondrial, and telomere length changes during short duration spaceflight","abstract":"Background: The Inspiration4 (I4) mission, the first all-civilian orbital flight mission, investigated the physiological effects of short-duration spaceflight through a multi-omic approach. Despite advances, there remains much to learn about human adaptation to spaceflight's unique challenges, including microgravity, immune system perturbations, and radiation exposure. Methods: To provide a detailed genetics analysis of the mission, we collected dried blood spots pre-, during, and post-flight for DNA extraction. Telomere length was measured by quantitative PCR, while whole genome and cfDNA sequencing provided insight into genomic stability and immune adaptations. A robust bioinformatic pipeline was used for data analysis, including variant calling to assess mutational burden. Result: Telomere elongation occurred during spaceflight and shortened after return to Earth. Cell-free DNA analysis revealed increased immune cell signatures post-flight. No significant clonal hematopoiesis of indeterminate potential (CHIP) or whole-genome instability was observed. The long-term gene expression changes across immune cells suggested cellular adaptations to the space environment persisting months post-flight. Conclusion: Our findings provide valuable insights into the physiological consequences of short-duration spaceflight, with telomere dynamics and immune cell gene expression adapting to spaceflight and persisting after return to Earth. CHIP sequencing data will serve as a reference point for studying the early development of CHIP in astronauts, an understudied phenomenon as previous studies have focused on career astronauts. This study will serve as a reference point for future commercial and non-commercial spaceflight, low Earth orbit (LEO) missions, and deep-space exploration.","journal":"Precision Clinical Medicine","year":2024,"id":429765,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":24,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9612,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":583411,"name":"Karolina Sienkiewicz","orcid":"0009-0004-7424-8989","position":1,"is_corresponding":false},{"id":853242,"name":"S Narayanan","orcid":"0000-0002-9783-5711","position":2,"is_corresponding":false},{"id":1163544,"name":"Eliah Overbey","orcid":"0000-0002-2866-8294","position":3,"is_corresponding":false},{"id":324399,"name":"Kirill Grigorev","orcid":"0000-0003-3628-0123","position":4,"is_corresponding":false},{"id":550379,"name":"Krista Ryon","orcid":"0000-0001-9440-2729","position":5,"is_corresponding":false},{"id":1205760,"name":"Marissa Burke","orcid":"0000-0001-5647-3358","position":6,"is_corresponding":false},{"id":1167343,"name":"Jacqueline Proszynski","orcid":null,"position":7,"is_corresponding":false},{"id":480729,"name":"Braden Tierney","orcid":"0000-0002-7533-8802","position":8,"is_corresponding":false},{"id":474307,"name":"Caleb M. Schmidt","orcid":null,"position":9,"is_corresponding":false},{"id":1232437,"name":"Nuria Mencia-Trinchant","orcid":"0000-0003-1376-8708","position":10,"is_corresponding":false},{"id":404913,"name":"Rémi Klotz","orcid":"0000-0003-2100-0635","position":11,"is_corresponding":false},{"id":995508,"name":"Veronica Ortiz","orcid":"0000-0002-6928-8458","position":12,"is_corresponding":false},{"id":227778,"name":"Jonathan Foox","orcid":"0000-0002-5705-3985","position":13,"is_corresponding":false},{"id":237408,"name":"Christopher R. Chin","orcid":"0000-0002-2140-3197","position":14,"is_corresponding":false},{"id":1167078,"name":"Deena Najjar","orcid":"0009-0009-7950-2866","position":15,"is_corresponding":false},{"id":324403,"name":"Irina Matei","orcid":"0000-0002-5712-8430","position":16,"is_corresponding":false},{"id":849570,"name":"Irenaeus C.C. Chan","orcid":"0009-0004-3250-9626","position":17,"is_corresponding":false},{"id":230680,"name":"Carlos Cruchaga","orcid":"0000-0002-0276-2899","position":18,"is_corresponding":false},{"id":1167341,"name":"Ashley S. Kleinman","orcid":null,"position":19,"is_corresponding":false},{"id":1163545,"name":"JangKeun Kim","orcid":"0000-0002-8733-9925","position":20,"is_corresponding":false},{"id":564564,"name":"Alexander G. Lucaci","orcid":"0000-0002-4896-6088","position":21,"is_corresponding":false},{"id":1163935,"name":"Conor Loy","orcid":null,"position":22,"is_corresponding":false},{"id":726281,"name":"Omary Mzava","orcid":null,"position":23,"is_corresponding":false},{"id":94489,"name":"Iwijn De Vlaminck","orcid":"0000-0001-6085-7311","position":24,"is_corresponding":false},{"id":91843,"name":"Anvita Singaraju","orcid":"0000-0002-3109-6354","position":25,"is_corresponding":false},{"id":324404,"name":"Lynn Taylor","orcid":"0000-0002-2074-7262","position":26,"is_corresponding":false},{"id":708136,"name":"Julian C. Schmidt","orcid":null,"position":27,"is_corresponding":false},{"id":473520,"name":"Michael A. Schmidt","orcid":"0000-0001-8765-5306","position":28,"is_corresponding":false},{"id":1173742,"name":"Kelly Blease","orcid":null,"position":29,"is_corresponding":false},{"id":1173743,"name":"Juan Moreno","orcid":null,"position":30,"is_corresponding":false},{"id":1173315,"name":"Andrew M. Boddicker","orcid":"0000-0001-7957-8283","position":31,"is_corresponding":false},{"id":294123,"name":"Junhua Zhao","orcid":"0009-0006-7672-1084","position":32,"is_corresponding":false},{"id":43053,"name":"Bryan R. Lajoie","orcid":"0000-0002-1895-8896","position":33,"is_corresponding":false},{"id":1173744,"name":"Andrew Altomare","orcid":null,"position":34,"is_corresponding":false},{"id":1173316,"name":"Semyon Kruglyak","orcid":"0000-0002-8353-1506","position":35,"is_corresponding":false},{"id":43059,"name":"Shawn Levy","orcid":"0000-0002-1369-5740","position":36,"is_corresponding":false},{"id":236061,"name":"Min Yu","orcid":"0000-0002-3969-8720","position":37,"is_corresponding":false},{"id":227768,"name":"Duane C. Hassane","orcid":"0000-0002-0719-9572","position":38,"is_corresponding":false},{"id":227785,"name":"Susan M. Bailey","orcid":"0000-0001-5595-9364","position":39,"is_corresponding":false},{"id":108786,"name":"Kelly L. Bolton","orcid":"0000-0001-6584-3357","position":40,"is_corresponding":false},{"id":1167345,"name":"Jaime Mateus","orcid":null,"position":41,"is_corresponding":false},{"id":36370,"name":"Christopher E. Mason","orcid":"0000-0002-1850-1642","position":42,"is_corresponding":false},{"id":1206610,"name":"J. Sebastian Garcia-Medina","orcid":null,"position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":null,"created_at":"2026-07-19T01:59:11.328802Z","pmid":"38634106","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}