{"doi":"10.1093/pcmedi/pbaa037","title":"Vegetarian diets, circulating miRNA expression and healthspan in subjects living in the Blue Zone","abstract":"A long-term vegetarian diet plays a role in the longevity and maintenance of the healthspan, but the underlying mechanisms for these observations are largely unknown. Particularly, it is not known whether a long-term vegetarian dietary pattern may affect the circulating miRNA expression in such a way as to modulate the healthspan. The Adventist Health Study-2 (AHS-2) cohort includes a large number of older adults who primarily follow vegetarian dietary patterns and reside in Loma Linda, California, one of five \"Blue Zones\" in the world in which a higher proportion of the population enjoys a longer than average lifespan. We performed miRNA-seq in 96 subjects selected from the AHS-2 cohort with different dietary patterns. We identified several differentially expressed miRNAs between vegetarians and non-vegetarians, which are involved in immune response and cytokine signaling, cell growth and proliferation as well as age-related diseases such as cardiovascular diseases and neurodegenerative diseases. Overall, our study showed that a vegetarian diet modulates aging-associated circulating miRNAs in a sex-dependent manner of differential expression for certain miRNAs, which may be related in a beneficial manner to the healthspan. Further investigation is needed to validate these miRNAs as potential biomarkers for diet-modulated longevity in humans.","journal":"Precision Clinical Medicine","year":2020,"id":103519,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":25,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9189,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":504376,"name":"Nicole M. Gatto","orcid":"0000-0001-7873-8310","position":1,"is_corresponding":false},{"id":504377,"name":"Zhong Chen","orcid":"0000-0002-3618-5073","position":2,"is_corresponding":false},{"id":320450,"name":"Hongyu Qiu","orcid":"0000-0002-2436-8921","position":3,"is_corresponding":false},{"id":504378,"name":"Grace Lee","orcid":"0000-0002-3123-0461","position":4,"is_corresponding":false},{"id":360826,"name":"Penelope J. Duerksen-Hughes","orcid":"0000-0002-0998-2157","position":5,"is_corresponding":false},{"id":270568,"name":"Gary E. Fraser","orcid":"0000-0002-5577-3524","position":6,"is_corresponding":false},{"id":24562,"name":"Charles Wang","orcid":null,"position":7,"is_corresponding":false},{"id":299070,"name":"Tiantian Liu","orcid":"0000-0001-9377-9938","position":0,"is_corresponding":true}],"reference_count":69,"raw_metadata":null,"created_at":"2026-07-18T22:42:48.999009Z","pmid":"33391847","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}