{"doi":"10.1093/noajnl/vdac131","title":"Generation of chromosome 1p/19q co-deletion by CRISPR/Cas9-guided genomic editing","abstract":"Background: Chromosomal translocation has been detected in many human cancers including gliomas and is considered a driving force in tumorigenesis. Co-deletion of chromosome arms 1p and 19q is a hallmark for oligodendrogliomas. On the molecular level, 1p/19q co-deletion results from t(1;19)(q10;p10), which leads to the concomitant formation of a hybrid chromosome containing the 1q and 19p arms. A method to generate 1p/19q co-deletion is lacking, which hinders the investigation of how 1p/19q co-deletion contributes to gliomagenesis. Methods: We hypothesized that chromosomal translocation, such as t(1;19)(q10;p10) resulting in the 1p/19q co-deletion, may be induced by simultaneously introducing DNA double-strand breaks (DSBs) into chromosomes 1p and 19q using CRISPR/Cas9. We developed a CRISPR/Cas9-based strategy to induce t(1;19)(q10;p10) and droplet digital PCR (ddPCR) assays to detect the hybrid 1q/19p and 1p/19q chromosomes. Results: After translocation induction, we detected both 1p/19q and 1q/19p hybrid chromosomes by PCR amplification of the junction regions in HEK 293T, and U-251 and LN-229 glioblastoma cells. Sequencing analyses of the PCR products confirmed DNA sequences matching both chromosomes 1 and 19. Furthermore, the 1p/19q hybrid chromosome was rapidly lost in all tested cell lines. The 1q/19p hybrid chromosome also become undetectable over time likely due to cell survival disadvantage. Conclusion: We demonstrated that t(1;19)(q10;p10) may be induced by CRISPR/Cas9-mediated genomic editing. This method represents an important step toward engineering the 1p/19q co-deletion to model oligodendrogliomas. This method may also be generalizable to engineering other cancer-relevant translocations, which may facilitate the understanding of translocation roles in cancer progression.","journal":"Neuro-Oncology Advances","year":2022,"id":276893,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9509,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":544303,"name":"Zhong Liu","orcid":"0000-0002-5132-7463","position":1,"is_corresponding":false},{"id":418667,"name":"Xiaoxia Zhang","orcid":"0009-0003-7031-9260","position":2,"is_corresponding":false},{"id":947864,"name":"Huafeng Wang","orcid":"0000-0002-3902-7054","position":3,"is_corresponding":false},{"id":242516,"name":"Gregory K. Friedman","orcid":"0000-0002-6653-7420","position":4,"is_corresponding":false},{"id":381132,"name":"Qiang Ding","orcid":"0000-0002-6226-869X","position":5,"is_corresponding":false},{"id":424479,"name":"Xinyang Zhao","orcid":"0000-0001-6677-7072","position":6,"is_corresponding":false},{"id":31912,"name":"Hu Li","orcid":"0000-0001-5957-5472","position":7,"is_corresponding":false},{"id":544304,"name":"Kitai Kim","orcid":"0000-0001-9782-9429","position":8,"is_corresponding":false},{"id":658329,"name":"Xi Yu","orcid":"0000-0003-0818-1451","position":9,"is_corresponding":false},{"id":225592,"name":"Burt Nabors","orcid":"0000-0001-5926-2230","position":10,"is_corresponding":false},{"id":453098,"name":"Xiaosi Han","orcid":"0000-0003-2985-8580","position":11,"is_corresponding":false},{"id":88476,"name":"Rui Zhao","orcid":"0000-0003-3769-6550","position":12,"is_corresponding":false},{"id":799233,"name":"Chao Li","orcid":"0000-0002-1950-0190","position":0,"is_corresponding":true}],"reference_count":42,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:28:34.768552Z","pmid":"36225650","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}