{"doi":"10.1093/neuonc/noaf201.0255","title":"CNSC-47. REAL-TIME CORRELATIVE MRI AND INTRAVITAL MICROSCOPY REVEAL MICROVASCULAR SIGNATURES IN BRAIN METASTASES","abstract":"Abstract Understanding brain vasculature in metastases requires correlating MRI resolution limitations with microscopic ground truth. Using the BRIDGE framework, we correlated two-photon (2P) microscopy with MRI (T2, T2*, TOF) to analyze vessel visibility. High-resolution 3D T2w imaging (40 µm) enabled visualization of vessels down to ~20 µm, revealing a significant inverse correlation between vessel volume density and T2w intensity. TOF sequences reflected blood flow velocity but not vessel diameter, while T2w sequences captured vascular structure. In a breast cancer brain metastases model (Jimt1), ex vivo MRI identified progressive T2*-hypointense lesions. Our in vivo BRIDGE pipeline linked T2* positivity to reduced blood flow velocity in perimetastatic capillaries <12 µm. Ex vivo analysis confirmed increased metastatic and erythrocyte clustering, with electron microscopy revealing vascular stasis. These findings validate MRI-based vascular signatures at microscopic resolution, highlighting microvascular dysfunction as a key factor in metastatic progression.","journal":"Neuro-Oncology","year":2025,"id":580671,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9511,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1491831,"name":"Julian Schroers","orcid":"0009-0001-9838-4801","position":1,"is_corresponding":false},{"id":1491832,"name":"Ekin Reyhan","orcid":"0000-0002-2109-0186","position":2,"is_corresponding":false},{"id":475112,"name":"Matthia A. Karreman","orcid":"0000-0003-0074-5945","position":3,"is_corresponding":false},{"id":1492140,"name":"Tara Mosigeh","orcid":null,"position":4,"is_corresponding":false},{"id":383582,"name":"Felix T. Kurz","orcid":"0000-0001-7616-7147","position":5,"is_corresponding":false},{"id":1013981,"name":"Varun Venkataramani","orcid":"0000-0002-2927-0886","position":6,"is_corresponding":false},{"id":1491830,"name":"Yvonne Yang","orcid":"0000-0001-7116-6911","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:58:43.046112Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}