{"doi":"10.1093/neuonc/noab023","title":"A serum-based DNA methylation assay provides accurate detection of glioma","abstract":"BACKGROUND: The detection of somatic mutations in cell-free DNA (cfDNA) from liquid biopsy has emerged as a noninvasive tool to monitor the follow-up of cancer patients. However, the significance of cfDNA clinical utility remains uncertain in patients with brain tumors, primarily because of the limited sensitivity cfDNA has to detect real tumor-specific somatic mutations. This unresolved challenge has prevented accurate follow-up of glioma patients with noninvasive approaches. METHODS: Genome-wide DNA methylation profiling of tumor tissue and serum cfDNA of glioma patients. RESULTS: Here, we developed a noninvasive approach to profile the DNA methylation status in the serum of patients with gliomas and identified a cfDNA-derived methylation signature that is associated with the presence of gliomas and related immune features. By testing the signature in an independent discovery and validation cohorts, we developed and verified a score metric (the \"glioma-epigenetic liquid biopsy score\" or GeLB) that optimally distinguished patients with or without glioma (sensitivity: 100%, specificity: 97.78%). Furthermore, we found that changes in GeLB score reflected clinicopathological changes during surveillance (eg, progression, pseudoprogression, and response to standard or experimental treatment). CONCLUSIONS: Our results suggest that the GeLB score can be used as a complementary approach to diagnose and follow up patients with glioma.","journal":"Neuro-Oncology","year":2021,"id":150751,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":89,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.957,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":496148,"name":"Tathiane M. Malta","orcid":"0000-0003-1129-5791","position":1,"is_corresponding":false},{"id":462106,"name":"James M. Snyder","orcid":"0000-0002-1386-2844","position":2,"is_corresponding":false},{"id":532411,"name":"Kevin Nelson","orcid":null,"position":3,"is_corresponding":false},{"id":536641,"name":"Michael Wells","orcid":"0000-0002-2592-2162","position":4,"is_corresponding":false},{"id":567148,"name":"Ana C. deCarvalho","orcid":"0000-0003-1183-4548","position":5,"is_corresponding":false},{"id":567149,"name":"Abir Mukherjee","orcid":"0000-0001-5234-0136","position":6,"is_corresponding":false},{"id":488696,"name":"Dhananjay Chitale","orcid":"0000-0002-4421-6431","position":7,"is_corresponding":false},{"id":567945,"name":"Maritza Mosella","orcid":null,"position":8,"is_corresponding":false},{"id":483573,"name":"Artem Sokolov","orcid":"0000-0002-8056-0504","position":9,"is_corresponding":false},{"id":567146,"name":"Karam Asmaro","orcid":"0000-0002-2084-4863","position":10,"is_corresponding":false},{"id":567150,"name":"Adam Robin","orcid":"0000-0003-4285-2722","position":11,"is_corresponding":false},{"id":567946,"name":"Mark L. Rosenblum","orcid":null,"position":12,"is_corresponding":false},{"id":13811,"name":"Tom Mikkelsen","orcid":"0000-0001-6285-2442","position":13,"is_corresponding":false},{"id":567151,"name":"Jack Rock","orcid":"0009-0000-1768-4195","position":14,"is_corresponding":false},{"id":79896,"name":"Laila Poisson","orcid":"0000-0002-3409-6536","position":15,"is_corresponding":false},{"id":363494,"name":"Ian Lee","orcid":"0000-0001-6057-7176","position":16,"is_corresponding":false},{"id":456434,"name":"Tobias Walbert","orcid":"0000-0003-0667-8106","position":17,"is_corresponding":false},{"id":70201,"name":"Steven N. Kalkanis","orcid":"0000-0001-5310-2682","position":18,"is_corresponding":false},{"id":51782,"name":"Antonio Iavarone","orcid":"0000-0002-0683-4634","position":19,"is_corresponding":false},{"id":640768,"name":"Ana Valeria Castro","orcid":"0000-0003-1625-0015","position":20,"is_corresponding":false},{"id":1835,"name":"Houtan Noushmehr","orcid":"0000-0003-4051-8114","position":21,"is_corresponding":false},{"id":567147,"name":"Thaís S. Sabedot","orcid":"0000-0002-7813-483X","position":0,"is_corresponding":true}],"reference_count":47,"raw_metadata":null,"created_at":"2026-07-18T23:43:06.501849Z","pmid":"33560371","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}