{"doi":"10.1093/ndt/gft345","title":"Determination of uromodulin in human urine: influence of storage and processing","abstract":null,"journal":"Nephrology Dialysis Transplantation","year":2014,"id":600312,"datarank":0.6937459219926407,"base_score":4.624972813284271,"endowment":4.624972813284271,"self_citation_contribution":0.6937459219926407,"citation_network_contribution":0.0,"self_endowment_contribution":0.6937459219926407,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":101,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":472276,"name":"Julien Weber","orcid":null,"position":1,"is_corresponding":false},{"id":1538988,"name":"Viviane Beaujean","orcid":null,"position":2,"is_corresponding":false},{"id":1538989,"name":"Bob Glaudemans","orcid":null,"position":3,"is_corresponding":false},{"id":1538990,"name":"Jens Sobek","orcid":null,"position":4,"is_corresponding":false},{"id":259790,"name":"Olivier Devuyst","orcid":"0000-0003-3744-4767","position":5,"is_corresponding":false},{"id":914122,"name":"Sonia Youhanna","orcid":"0000-0002-9376-8478","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Determination of uromodulin in human urine: influence of storage and processing","abstract":"BACKGROUND: Uromodulin (Tamm-Horsfall protein) is the most abundant protein excreted in the urine under physiological conditions. It is exclusively produced in the kidney and secreted into the urine via proteolytic cleavage. The involvement of UMOD, the gene that encodes uromodulin, in rare autosomal dominant diseases, and its robust genome-wide association with the risk of chronic kidney disease suggest that the level of uromodulin in urine could represent a critical biomarker for kidney function. The structure of uromodulin is complex, with multiple disulfide bonds and typical domains of extracellular proteins. METHODS: Thus far, the conditions influencing stability and measurement of uromodulin in human urine have not been systematically investigated, giving inconsistent results. In this study, we used a robust, in-house ELISA to characterize the conditions of sampling and storage necessary to provide a faithful dosage of uromodulin in the urine. RESULTS: The levels of uromodulin in human urine were significantly affected by centrifugation and vortexing, as well as by the conditions and duration of storage. CONCLUSIONS: These results validate a simple, low-cost ELISA and document the optimal conditions of processing and storage for measuring uromodulin in human urine.","is_dataset_classified":null,"base_score":4.624972813284271,"endowment":4.624972813284271,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24097801","pmcid":null,"openalex_id":"https://openalex.org/W2096545844","authors":[],"funders":[{"funder_name":"European Commission","grant_id":"246539","title":"Postdoctoral Program in Integrative Kidney Physiology and Pathophysiology"},{"funder_name":"European Commission","grant_id":"305608","title":"European Consortium for High-Throughput Research in Rare Kidney Diseases"}],"total_grants":2,"fwci":3.0548,"citation_percentile":0.9177235,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":2},{"year":2015,"count":6},{"year":2016,"count":5},{"year":2017,"count":8},{"year":2018,"count":10},{"year":2019,"count":8},{"year":2020,"count":7},{"year":2021,"count":9},{"year":2022,"count":12},{"year":2023,"count":10},{"year":2024,"count":9},{"year":2025,"count":9},{"year":2026,"count":5}],"oa_status":"bronze","license":"http://rightsstatements.org/page/InC-NC/1.0/","oa_locations":[{"url":"https://academic.oup.com/ndt/article-pdf/29/1/136/12762104/gft345.pdf","host_type":"journal"},{"url":"https://academic.oup.com/ndt/article-pdf/29/1/136/12762104/gft345.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/ndt/article-pdf/29/1/136/12762104/gft345.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/ndt/gft345","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24097801","host_type":"repository"},{"url":"http://hdl.handle.net/2078.1/157097","host_type":"repository"},{"url":"http://hdl.handle.net/20.500.11850/78944","host_type":"repository"},{"url":"https://doi.org/10.3929/ethz-b-000078944","host_type":"repository"},{"url":"https://doi.org/10.5167/uzh-83925","host_type":""},{"url":"https://dx.doi.org/10.5167/uzh-83925","host_type":""},{"url":"https://dx.doi.org/10.3929/ethz-b-000078944","host_type":""},{"url":"http://dx.doi.org/10.1093/ndt/gft345","host_type":""},{"url":"https://dx.doi.org/10.1093/ndt/gft345","host_type":""},{"url":"https://sonar.ch/global/documents/65997","host_type":""},{"url":"https://www.zora.uzh.ch/id/eprint/83925/","host_type":""}],"fields_of_study":["Kidney Stones and Urolithiasis Treatments","Chronic Kidney Disease and Diabetes","Bladder and Urothelial Cancer Treatments","03 medical and health sciences","0302 clinical medicine","Adolescent","Adult","Animals","Biomarkers","Centrifugation","Enzyme-Linked Immunosorbent Assay","Female","Gout","Humans","Hyperuricemia","Kidney","Kidney Diseases","Male","Middle Aged","Specimen Handling","Temperature","Uromodulin","Young Adult","Juvenile gout"],"mesh_terms":["Adolescent","Adult","Animals","Centrifugation","Enzyme-Linked Immunosorbent Assay","Female","Gout","Humans","Kidney","Kidney Diseases","Male","Middle Aged","Specimen Handling","Temperature","Biomarkers","Hyperuricemia","Young Adult","Uromodulin"],"keywords":["Tamm–Horsfall protein","Urine","Medicine","Kidney","Biomarker","Kidney disease","Urinary system","Endocrinology","Biology","Biochemistry","ELISA","Uromodulin-associated Kidney Disease","Adult","Male","Adolescent","Gout","2747 Transplantation","610 Medicine & health","10071 Functional Genomics Center Zurich","Centrifugation","Enzyme-Linked Immunosorbent Assay","Hyperuricemia","10052 Institute of Physiology","Specimen Handling","Young Adult","11554 Zurich Center for Integrative Human Physiology (ZIHP)","Uromodulin","Animals","Humans","610 Medicine & health","2727 Nephrology","Temperature","Middle Aged","570 Life sciences; biology","Female","Kidney Diseases","Biomarkers"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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