{"doi":"10.1093/nass/nrm024","title":"Chemistry of Bisulfite Genomic Sequencing; Advances and Issues","abstract":null,"journal":"Nucleic Acids Symposium Series","year":2007,"id":674301,"datarank":0.24141568686511508,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1509191,"name":"K. Negishi","orcid":null,"position":1,"is_corresponding":false},{"id":1761753,"name":"M. Shiraishi","orcid":null,"position":2,"is_corresponding":false},{"id":137166,"name":"K. Tsuji","orcid":null,"position":3,"is_corresponding":false},{"id":1761756,"name":"K. Moriyama","orcid":null,"position":4,"is_corresponding":false},{"id":1761752,"name":"H. Hayatsu","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Chemistry of Bisulfite Genomic Sequencing; Advances and Issues","abstract":"Methylation at position 5 of cytosine in DNA plays a major role in epigenetic gene control. The methylation analysis can be performed by bisulfite genomic sequencing. Conventional procedures in this analysis include a treatment of single stranded DNA with 3-5 M sodium bisulfite at pH 5 and at 50-55 degrees for 4-20 hr. This will convert cytosine into uracil, while 5-methylcytosine resists this deamination. Amplification by PCR of the bisulfite-treated DNA followed by sequencing reveals the positions of 5-methylcytosine in the gene. We reported recently that the whole procedure can be speeded up by use of a highly concentrated bisulfite solution, 10 M ammonium bisulfite. We also reported that urea, which has been often added to the reaction mixture with the purpose of facilitating the reaction, may not work as anticipated. This time, we would like to address the need for further investigating the chemistry of the bisulfite modification of DNA. Particularly important is to study side reactions that may occur due to the exhaustive bisulfite treatment required for achieving complete deamination of all the cytosine residues in a given sample of DNA.","is_dataset_classified":null,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18029579","pmcid":null,"openalex_id":"https://openalex.org/W2087999257","authors":[],"funders":[],"total_grants":0,"fwci":1.6531,"citation_percentile":0.83323389,"influential_citations":0,"citation_trend":[{"year":2015,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://academic.oup.com/nass/article-pdf/51/1/47/3376963/nrm024.pdf","host_type":"journal"},{"url":"https://academic.oup.com/nass/article-pdf/51/1/47/3376963/nrm024.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/nass/article-pdf/51/1/47/3376963/nrm024.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/nass/nrm024","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/18029579","host_type":"repository"}],"fields_of_study":["Epigenetics and DNA Methylation","Cancer-related gene regulation","RNA modifications and cancer","5-Methylcytosine","Cell Line, Tumor","Cytosine","DNA Methylation","Deamination","Genomics","Humans","Quaternary Ammonium Compounds","Sequence Analysis, DNA","Sulfites","Urea"],"mesh_terms":["Quaternary Ammonium Compounds","Cytosine","Deamination","Humans","Sulfites","Urea","Sequence Analysis, DNA","DNA Methylation","Genomics","5-Methylcytosine","Cell Line, Tumor"],"keywords":["Bisulfite","Deamination","Sodium bisulfite","Bisulfite sequencing","Cytosine","Methylated DNA immunoprecipitation","DNA methylation","Chemistry","DNA","5-Methylcytosine","Biochemistry","Molecular biology","Computational biology","Gene","Biology","Organic chemistry","Gene expression"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T16:23:45.047864Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}