{"doi":"10.1093/nargab/lqaf066","title":"VariantFoldRNA: a flexible, containerized, and scalable pipeline for genome-wide riboSNitch prediction","abstract":"Single nucleotide polymorphisms (SNPs) can alter RNA structure by changing the proportions of existing conformations or leading to new conformations in the structural ensemble. Such structure-changing SNPs, or riboSNitches, have been associated with diseases in humans and climate adaptation in plants. While several computational tools are available for predicting whether an SNP is a riboSNitch, these tools were generally developed to analyze individual RNAs and are not optimized for genome-wide analyses. To fill this gap, we developed VariantFoldRNA, a flexible, containerized, and automated pipeline for genome-wide prediction of riboSNitches. Our pipeline automatically installs all dependencies, can be run locally or on high-performance clusters, and is modular, enabling the user to customize the analysis for the research question of interest. VariantFoldRNA can predict riboSNitches genome-wide at user-specified temperatures and splicing conditions, opening the door to novel analyses. The pipeline is an open-source command-line tool that is freely available at https://github.com/The-Bevilacqua-Lab/variantfoldrna.","journal":"NAR Genomics and Bioinformatics","year":2025,"id":567036,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9557,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":406497,"name":"Philip C. Bevilacqua","orcid":"0000-0001-8074-3434","position":1,"is_corresponding":false},{"id":406496,"name":"Sarah M. Assmann","orcid":"0000-0003-4541-1594","position":2,"is_corresponding":false},{"id":1235137,"name":"Kobie J. Kirven","orcid":"0000-0002-8627-0743","position":0,"is_corresponding":true}],"reference_count":35,"raw_metadata":null,"created_at":"2026-07-19T02:56:40.491968Z","pmid":"40443739","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}