{"doi":"10.1093/nargab/lqac065","title":"A high-throughput 384-well CometChip platform reveals a role for 3-methyladenine in the cellular response to etoposide-induced DNA damage","abstract":"The Comet or single-cell gel electrophoresis assay is a highly sensitive method to measure cellular, nuclear genome damage. However, low throughput can limit its application for large-scale studies. To overcome these limitations, a 96-well CometChip platform was recently developed that increases throughput and reduces variation due to simultaneous processing and automated analysis of 96 samples. To advance throughput further, we developed a 384-well CometChip platform that allows analysis of ∼100 cells per well. The 384-well CometChip extends the capacity by 4-fold as compared to the 96-well system, enhancing application for larger DNA damage analysis studies. The overall sensitivity of the 384-well CometChip is consistent with that of the 96-well system, sensitive to genotoxin exposure and to loss of DNA repair capacity. We then applied the 384-well platform to screen a library of protein kinase inhibitors to probe each as enhancers of etoposide induced DNA damage. Here, we found that 3-methyladenine significantly increased levels of etoposide-induced DNA damage. Our results suggest that a 384-well CometChip is useful for large-scale DNA damage analyses, which may have increased potential in the evaluation of chemotherapy efficacy, compound library screens, population-based analyses of genome damage and evaluating the impact of environmental genotoxins on genome integrity.","journal":"NAR Genomics and Bioinformatics","year":2022,"id":276984,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9586,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":693664,"name":"Alison Beiser","orcid":"0000-0001-5295-8454","position":1,"is_corresponding":false},{"id":947964,"name":"Nupur B. Dey","orcid":"0000-0002-5504-2115","position":2,"is_corresponding":false},{"id":329462,"name":"Shunichi Takeda","orcid":"0000-0002-7924-7991","position":3,"is_corresponding":false},{"id":278617,"name":"Liton Kumar Saha","orcid":"0000-0003-3391-280X","position":4,"is_corresponding":false},{"id":483352,"name":"Kouji Hirota","orcid":"0000-0003-1676-979X","position":5,"is_corresponding":false},{"id":947965,"name":"L. Lynette Parker","orcid":"0000-0002-3535-3362","position":6,"is_corresponding":false},{"id":947966,"name":"Mariah Carter","orcid":"0000-0002-1364-4994","position":7,"is_corresponding":false},{"id":947967,"name":"Martha I. Arrieta","orcid":"0000-0003-2514-5743","position":8,"is_corresponding":false},{"id":285722,"name":"Robert W. Sobol","orcid":"0000-0001-7385-3563","position":9,"is_corresponding":false},{"id":285713,"name":"Jianfeng Li","orcid":"0000-0003-2349-208X","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":null,"created_at":"2026-07-19T00:28:34.768552Z","pmid":"36110898","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}