{"doi":"10.1093/narcan/zcad021","title":"Characterization of cell-type specific circular RNAs associated with colorectal cancer metastasis","abstract":"Colorectal cancer (CRC) is the most common gastrointestinal malignancy and a leading cause of cancer deaths in the United States. More than half of CRC patients develop metastatic disease (mCRC) with an average 5-year survival rate of 13%. Circular RNAs (circRNAs) have recently emerged as important tumorigenesis regulators; however, their role in mCRC progression remains poorly characterized. Further, little is known about their cell-type specificity to elucidate their functions in the tumor microenvironment (TME). To address this, we performed total RNA sequencing (RNA-seq) on 30 matched normal, primary and metastatic samples from 14 mCRC patients. Additionally, five CRC cell lines were sequenced to construct a circRNA catalog in CRC. We detected 47 869 circRNAs, with 51% previously unannotated in CRC and 14% novel candidates when compared to existing circRNA databases. We identified 362 circRNAs differentially expressed in primary and/or metastatic tissues, termed circular RNAs associated with metastasis (CRAMS). We performed cell-type deconvolution using published single-cell RNA-seq datasets and applied a non-negative least squares statistical model to estimate cell-type specific circRNA expression. This predicted 667 circRNAs as exclusively expressed in a single cell type. Collectively, this serves as a valuable resource, TMECircDB (accessible at https://www.maherlab.com/tmecircdb-overview), for functional characterization of circRNAs in mCRC, specifically in the TME.","journal":"NAR Cancer","year":2023,"id":351167,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9541,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":873608,"name":"Amy Ly","orcid":"0000-0002-4888-5026","position":1,"is_corresponding":false},{"id":266772,"name":"Jacqueline L. Mudd","orcid":"0000-0001-7618-8765","position":2,"is_corresponding":false},{"id":266767,"name":"Emily B. Rozycki","orcid":"0009-0008-4651-0176","position":3,"is_corresponding":false},{"id":1095864,"name":"Jace Webster","orcid":"0000-0002-9396-5658","position":4,"is_corresponding":false},{"id":506483,"name":"Emily Coonrod","orcid":"0000-0003-0877-6236","position":5,"is_corresponding":false},{"id":506482,"name":"Ghofran Othoum","orcid":"0000-0002-0376-3244","position":6,"is_corresponding":false},{"id":2218,"name":"Jingqin Luo","orcid":"0000-0003-2759-3072","position":7,"is_corresponding":false},{"id":266763,"name":"Ha X. Dang","orcid":"0000-0002-9342-9249","position":8,"is_corresponding":false},{"id":31720,"name":"Ryan C. Fields","orcid":"0000-0001-6176-8943","position":9,"is_corresponding":false},{"id":266775,"name":"Christopher A. Maher","orcid":"0000-0002-9035-603X","position":10,"is_corresponding":false},{"id":235665,"name":"Sidi Zhao","orcid":"0000-0002-8375-9279","position":0,"is_corresponding":true}],"reference_count":98,"raw_metadata":null,"created_at":"2026-07-19T01:12:41.740237Z","pmid":"37213253","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}