{"doi":"10.1093/nar/gky1143","title":"Single-molecule imaging of DNA gyrase activity in living\n                    <i>Escherichia coli</i>","abstract":null,"journal":"Nucleic Acids Research","year":2019,"id":605299,"datarank":0.7037021823343717,"base_score":4.6913478822291435,"endowment":4.6913478822291435,"self_citation_contribution":0.7037021823343717,"citation_network_contribution":0.0,"self_endowment_contribution":0.7037021823343717,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":108,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1553391,"name":"Adam J M Wollman","orcid":null,"position":1,"is_corresponding":false},{"id":1553392,"name":"Elzbieta Kaja","orcid":null,"position":2,"is_corresponding":false},{"id":1553393,"name":"Jacek Gapinski","orcid":null,"position":3,"is_corresponding":false},{"id":436130,"name":"Ji-Eun Lee","orcid":"0000-0003-1708-4303","position":4,"is_corresponding":false},{"id":1553394,"name":"Victoria A Leek","orcid":null,"position":5,"is_corresponding":false},{"id":1553395,"name":"Shannon J McKie","orcid":null,"position":6,"is_corresponding":false},{"id":1553396,"name":"Lesley A Mitchenall","orcid":null,"position":7,"is_corresponding":false},{"id":397463,"name":"Anthony Maxwell","orcid":"0000-0002-5756-6430","position":8,"is_corresponding":false},{"id":1553397,"name":"David J Sherratt","orcid":null,"position":9,"is_corresponding":false},{"id":589777,"name":"Mark C. Leake","orcid":"0000-0002-1715-1249","position":10,"is_corresponding":false},{"id":241326,"name":"Paweł Zawadzki","orcid":"0000-0002-9032-2315","position":11,"is_corresponding":false},{"id":1553390,"name":"Mathew Stracy","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Single-molecule imaging of DNA gyrase activity in living\n                    <i>Escherichia coli</i>","abstract":"Bacterial DNA gyrase introduces negative supercoils into chromosomal DNA and relaxes positive supercoils introduced by replication and transiently by transcription. Removal of these positive supercoils is essential for replication fork progression and for the overall unlinking of the two duplex DNA strands, as well as for ongoing transcription. To address how gyrase copes with these topological challenges, we used high-speed single-molecule fluorescence imaging in live Escherichia coli cells. We demonstrate that at least 300 gyrase molecules are stably bound to the chromosome at any time, with ∼12 enzymes enriched near each replication fork. Trapping of reaction intermediates with ciprofloxacin revealed complexes undergoing catalysis. Dwell times of ∼2 s were observed for the dispersed gyrase molecules, which we propose maintain steady-state levels of negative supercoiling of the chromosome. In contrast, the dwell time of replisome-proximal molecules was ∼8 s, consistent with these catalyzing processive positive supercoil relaxation in front of the progressing replisome.","is_dataset_classified":null,"base_score":4.6913478822291435,"endowment":4.6913478822291435,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30445553","pmcid":"PMC6326794","openalex_id":"https://openalex.org/W2963402579","authors":[],"funders":[{"funder_name":"National Science Centre Poland","grant_id":"2015/19/P/NZ1/03859","title":null},{"funder_name":"Foundation for Polish Science","grant_id":"TEAM/2016-1/9","title":null},{"funder_name":"Medical Research Council","grant_id":"MwR/K01580X/1","title":null},{"funder_name":"Biotechnology and Biological Sciences Research Council","grant_id":"BB/N006453/1","title":"Replication repair in real life: analysing how broken DNA replication machines are rebuilt inside cells."},{"funder_name":"Biotechnology and Biological Sciences Research Council","grant_id":"BB/R001235/1","title":"Tackling tricky twists - how does DNA gyrase function inside living cells?"},{"funder_name":"Biotechnology and Biological Sciences Research Council","grant_id":"BB/J004561/1","title":null},{"funder_name":"Biotechnology and Biological Sciences Research Council","grant_id":"BB/P012523/1","title":null},{"funder_name":"University of York","grant_id":"204829","title":"Institutional Strategic Support Fund"},{"funder_name":"Wellcome Trust","grant_id":"099204/Z/12Z","title":null},{"funder_name":"Sir Henry Wellcome","grant_id":"204684/Z/16/Z","title":null},{"funder_name":"Leverhulme Trust","grant_id":"RP2013-K-017","title":null},{"funder_name":"Biotechnology and Biological Sciences Research Council","grant_id":"BB/R001243/1","title":null},{"funder_name":"Biotechnology and Biological Sciences Research Council","grant_id":"BBS/E/J/000PR9791","title":null},{"funder_name":"Wellcome Trust","grant_id":"099204","title":"Illuminating the in vivo molecular mechanism of bacterial chromosome replication and segregation."},{"funder_name":"Biotechnology and Biological Sciences Research Council","grant_id":"BBS/E/J/00000201","title":null},{"funder_name":"Wellcome Trust","grant_id":"200782/Z/16/Z","title":null},{"funder_name":"Medical Research Council","grant_id":"MR/K01580X/1","title":"MICA: Bio-Continuum Microscopy: Seamless imaging from the micro- to the nano-scale"},{"funder_name":"Wellcome Trust","grant_id":"204684","title":"The fate of multidrug tolerant bacteria: from single cells to microbial communities."},{"funder_name":"Fundação para a Ciência e a Tecnologia, I.P.","grant_id":"PTDC/CCI-BIO/29266/2017","title":"Deep Drug Discovery and Deployment"}],"total_grants":19,"fwci":3.8744,"citation_percentile":0.95002592,"influential_citations":0,"citation_trend":[{"year":2019,"count":14},{"year":2020,"count":18},{"year":2021,"count":15},{"year":2022,"count":12},{"year":2023,"count":14},{"year":2024,"count":14},{"year":2025,"count":17},{"year":2026,"count":4}],"oa_status":"gold","license":"cc-by-nc","oa_locations":[{"url":"https://academic.oup.com/nar/article-pdf/47/1/210/27457476/gky1143.pdf","host_type":"journal"},{"url":"https://academic.oup.com/nar/article-pdf/47/1/210/27457476/gky1143.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/nar/article-pdf/47/1/210/27457476/gky1143.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/nar/gky1143","host_type":"journal"},{"url":"https://europepmc.org/articles/PMC6326794","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC6326794?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.1101/460006","host_type":""},{"url":"https://dx.doi.org/10.48550/arxiv.1811.00531","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/30445553","host_type":""},{"url":"http://dx.doi.org/10.1093/nar/gky1143","host_type":""},{"url":"http://arxiv.org/abs/1811.00531","host_type":""},{"url":"https://eprints.whiterose.ac.uk/137893/21/gky1143.pdf","host_type":""},{"url":"https://dx.doi.org/10.1101/460006","host_type":""},{"url":"https://dx.doi.org/10.1093/nar/gky1143","host_type":""},{"url":"https://ora.ox.ac.uk/objects/uuid:96dceba5-b2c8-4947-9205-6dab99c98baa","host_type":""},{"url":"http://dx.doi.org/10.1101/460006","host_type":""},{"url":"https://doi.org/https://doi.org/10.1093/nar/gky1143","host_type":""}],"fields_of_study":["Cancer therapeutics and mechanisms","DNA Repair Mechanisms","DNA and Nucleic Acid Chemistry","0301 basic medicine","0303 health sciences","03 medical and health sciences","Catalysis","DNA Gyrase","DNA, Superhelical","DNA-Binding Proteins","Escherichia coli","Protein Binding","Single Molecule Imaging"],"mesh_terms":["Escherichia coli","DNA Gyrase","DNA-Binding Proteins","DNA, Superhelical","Protein Binding","Catalysis","Single Molecule Imaging"],"keywords":["DNA supercoil","DNA gyrase","Replisome","Biology","Circular bacterial chromosome","Nucleoid","DNA","Escherichia coli","DNA replication","Biophysics","Transcription (linguistics)","Molecular biology","Cell biology","Genetics","Gene","1311","DNA, Superhelical","FOS: Physical sciences","Biomolecules (q-bio.BM)","Genome Integrity, Repair and Replication","Catalysis","Single Molecule Imaging","DNA-Binding Proteins","Quantitative Biology - Biomolecules","Biological Physics (physics.bio-ph)","FOS: Biological sciences","Physics - Biological Physics","Protein Binding"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. Good health"},{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T02:06:46.682652Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}