{"doi":"10.1093/nar/gku560","title":"Inventory of telomerase components in human cells reveals multiple subpopulations of hTR and hTERT","abstract":null,"journal":"Nucleic Acids Research","year":2014,"id":47433,"datarank":4.892475700093268,"base_score":5.0369526024136295,"endowment":5.0369526024136295,"self_citation_contribution":0.7555428903620446,"citation_network_contribution":4.136932809731223,"self_endowment_contribution":0.7555428903620446,"citer_contribution":4.136932809731223,"corpus_percentile":null,"corpus_rank":null,"citation_count":153,"citer_count":138,"citers_with_citation_signal":116,"citers_with_endowment":116,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":218800,"name":"Thomas R. Cech","orcid":null,"position":1,"is_corresponding":false},{"id":219583,"name":"Linghe Xi","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Inventory of telomerase components in human cells reveals multiple subpopulations of hTR and hTERT","abstract":"Telomerase is the ribonucleoprotein (RNP) enzyme that elongates telomeric DNA to compensate for the attrition occurring during each cycle of DNA replication. Knowing the levels of telomerase in continuously dividing cells is important for understanding how much telomerase is required for cell immortality. In this study, we measured the endogenous levels of the human telomerase RNP and its two key components, human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT). We estimate ∼ 240 telomerase monomers per cell for HEK 293T and HeLa, a number similar to that of telomeres in late S phase. The subunits were in excess of RNPs (e.g. ∼ 1150 hTR and ∼ 500 hTERT molecules per HeLa cell), suggesting the existence of unassembled components. This hypothesis was tested by overexpressing individual subunits, which increased total telomerase activity as measured by the direct enzyme assay. Thus, there are subpopulations of both hTR and hTERT not assembled into telomerase but capable of being recruited. We also determined the specific activity of endogenous telomerase and of overexpressed super-telomerase both to be ∼ 60 nt incorporated per telomerase per minute, with Km(dGTP) ∼ 17 μM, indicating super-telomerase is as catalytically active as endogenous telomerase and is thus a good model for biochemical studies.","is_dataset_classified":null,"base_score":5.0369526024136295,"endowment":5.0369526024136295,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24990373","pmcid":"PMC4117779","openalex_id":"https://openalex.org/W2135532291","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM08759","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM142607","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM099705","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM008759","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01GM099705-06","title":"TERT Promoter Mutations and Telomerase Reactivation in Cancer Cells"},{"funder_name":"National Institutes of Health","grant_id":"5T32GM008759-05","title":"TRAINING IN SIGNAL TRANSDUCTION &CELL CYCLE REGULATION"},{"funder_name":"Howard Hughes Medical Institute","grant_id":"","title":null},{"funder_name":"Howard Hughes Medical Institute","grant_id":"","title":null}],"total_grants":8,"fwci":8.6501,"citation_percentile":0.98426224,"influential_citations":1,"citation_trend":[{"year":2014,"count":3},{"year":2015,"count":17},{"year":2016,"count":21},{"year":2017,"count":12},{"year":2018,"count":7},{"year":2019,"count":16},{"year":2020,"count":16},{"year":2021,"count":16},{"year":2022,"count":5},{"year":2023,"count":10},{"year":2024,"count":13},{"year":2025,"count":12},{"year":2026,"count":5}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://academic.oup.com/nar/article-pdf/42/13/8565/16941177/gku560.pdf","host_type":"journal"},{"url":"https://academic.oup.com/nar/article-pdf/42/13/8565/16941177/gku560.pdf","host_type":"GOLD"},{"url":"https://academic.oup.com/nar/article-pdf/42/13/8565/16941177/gku560.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/nar/article-pdf/42/13/8565/16941177/gku560.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/nar/gku560","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24990373","host_type":"repository"},{"url":"https://scholar.colorado.edu/concern/articles/bg257f83v","host_type":"repository"},{"url":"https://scholar.colorado.edu/libr_oafund/72","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.869.4182","host_type":""},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4117779","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4117779","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4117779?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1093/nar/gku560","host_type":""},{"url":"https://dx.doi.org/10.1093/nar/gku560","host_type":""}],"fields_of_study":["Telomeres, Telomerase, and Senescence","RNA Interference and Gene Delivery","Advanced biosensing and bioanalysis techniques","Biology","Medicine","0301 basic medicine","0303 health sciences","03 medical and health sciences","Blotting, Western","Cell Line","HEK293 Cells","HeLa Cells","Humans","Immunoprecipitation","RNA","Telomerase"],"mesh_terms":["Cell Line","HeLa Cells","Humans","RNA","Blotting, Western","Telomerase","Immunoprecipitation","HEK293 Cells","Hela Cells"],"keywords":["Telomerase","Telomerase reverse transcriptase","Telomere","Telomerase RNA component","Biology","Ribonucleoprotein","HeLa","Molecular biology","HEK 293 cells","Protein subunit","DNA","RNA","Cell","Cell biology","Cell culture","Biochemistry","Genetics","Gene","571","Blotting","Nucleic Acid Enzymes","Blotting, Western","Cell Line","HEK293 Cells","Humans","Immunoprecipitation","Western","HeLa Cells"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-18T17:02:01.045207Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}