{"doi":"10.1093/nar/gkt409","title":"UGA codon position-dependent incorporation of selenocysteine into mammalian selenoproteins","abstract":null,"journal":"Nucleic Acids Research","year":2013,"id":688455,"datarank":0.5456379239589579,"base_score":3.6375861597263857,"endowment":3.6375861597263857,"self_citation_contribution":0.5456379239589579,"citation_network_contribution":0.0,"self_endowment_contribution":0.5456379239589579,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":37,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1798540,"name":"A. V. Lobanov","orcid":null,"position":1,"is_corresponding":false},{"id":1798541,"name":"D. L. Hatfield","orcid":null,"position":2,"is_corresponding":false},{"id":1798542,"name":"V. N. Gladyshev","orcid":null,"position":3,"is_corresponding":false},{"id":1798539,"name":"A. A. Turanov","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"UGA codon position-dependent incorporation of selenocysteine into mammalian selenoproteins","abstract":"It is thought that the SelenoCysteine Insertion Sequence (SECIS) element and UGA codon are sufficient for selenocysteine (Sec) insertion. However, we found that UGA supported Sec insertion only at its natural position or in its close proximity in mammalian thioredoxin reductase 1 (TR1). In contrast, Sec could be inserted at any tested position in mammalian TR3. Replacement of the 3'-UTR of TR3 with the corresponding segment of a Euplotes crassus TR restricted Sec insertion into the C-terminal region, whereas the 3'-UTR of TR3 conferred unrestricted Sec insertion into E. crassus TR, in which Sec insertion is normally limited to the C-terminal region. Exchanges of 3'-UTRs between mammalian TR1 and E. crassus TR had no effect, as both proteins restricted Sec insertion. We further found that these effects could be explained by the use of selenoprotein-specific SECIS elements. Examination of Sec insertion into other selenoproteins was consistent with this model. The data indicate that mammals evolved the ability to limit Sec insertion into natural positions within selenoproteins, but do so in a selenoprotein-specific manner, and that this process is controlled by the SECIS element in the 3'-UTR.","is_dataset_classified":null,"base_score":3.6375861597263857,"endowment":3.6375861597263857,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23716634","pmcid":"PMC3737529","openalex_id":"https://openalex.org/W2169851348","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM065204","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R37 GM065204","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM061603","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM061603","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM065204","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01GM065204-04","title":"Functions of Mammalian thioredoxin reductases"},{"funder_name":"National Institutes of Health","grant_id":"2R01GM061603-10","title":"Identity and Function of Selenoprotein Genes"}],"total_grants":7,"fwci":2.3656,"citation_percentile":0.88123604,"influential_citations":0,"citation_trend":[{"year":2014,"count":5},{"year":2015,"count":2},{"year":2016,"count":4},{"year":2017,"count":5},{"year":2018,"count":3},{"year":2019,"count":3},{"year":2020,"count":1},{"year":2021,"count":5},{"year":2022,"count":3},{"year":2023,"count":1},{"year":2025,"count":4},{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://academic.oup.com/nar/article-pdf/41/14/6952/4906071/gkt409.pdf","host_type":"journal"},{"url":"https://academic.oup.com/nar/article-pdf/41/14/6952/4906071/gkt409.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/nar/article-pdf/41/14/6952/4906071/gkt409.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/nar/gkt409","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23716634","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3737529","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.788.9549","host_type":""},{"url":"http://nrs.harvard.edu/urn-3:HUL.InstRepos:11855815","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC3737529","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3737529?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1093/nar/gkt409","host_type":""},{"url":"https://dx.doi.org/10.1093/nar/gkt409","host_type":""},{"url":"https://doi.org/https://doi.org/10.1093/nar/gkt409","host_type":""}],"fields_of_study":["Selenium in Biological Systems","Trace Elements in Health","Redox biology and oxidative stress","0301 basic medicine","0303 health sciences","03 medical and health sciences","3' Untranslated Regions","Codon","HEK293 Cells","Humans","Selenocysteine","Selenoproteins"],"mesh_terms":["Codon","Humans","Selenocysteine","3' Untranslated Regions","Selenoproteins","HEK293 Cells"],"keywords":["Selenocysteine","Selenoprotein","Insertion sequence","Biology","Stop codon","Genetics","Selenoprotein P","Untranslated region","Thioredoxin reductase","Three prime untranslated region","Thioredoxin","Biochemistry","Amino acid","Gene","Messenger RNA","Cysteine","Mutant","Enzyme","Transposable element","Glutathione","Glutathione peroxidase","HEK293 Cells","Humans","Codon","Selenoproteins","Molecular Biology","3' Untranslated Regions"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-19T16:21:32.753053Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}