{"doi":"10.1093/nar/gks315","title":"Homologous recombination repairs secondary replication induced DNA double-strand breaks after ionizing radiation","abstract":null,"journal":"Nucleic Acids Research","year":2012,"id":679733,"datarank":0.6476232170304466,"base_score":4.31748811353631,"endowment":4.31748811353631,"self_citation_contribution":0.6476232170304466,"citation_network_contribution":0.0,"self_endowment_contribution":0.6476232170304466,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":74,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1776017,"name":"Manuel Luís Orta","orcid":null,"position":1,"is_corresponding":false},{"id":599665,"name":"Ingegerd Elvers","orcid":"0000-0002-6604-0369","position":2,"is_corresponding":false},{"id":1776018,"name":"Muntasir Mamun Majumder","orcid":null,"position":3,"is_corresponding":false},{"id":1776020,"name":"Anne Lagerqvist","orcid":null,"position":4,"is_corresponding":false},{"id":320616,"name":"Thomas Helleday","orcid":"0000-0002-7384-092X","position":5,"is_corresponding":false},{"id":1776016,"name":"Petra Groth","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Homologous recombination repairs secondary replication induced DNA double-strand breaks after ionizing radiation","abstract":"Ionizing radiation (IR) produces direct two-ended DNA double-strand breaks (DSBs) primarily repaired by non-homologous end joining (NHEJ). It is, however, well established that homologous recombination (HR) is induced and required for repair of a subset of DSBs formed following IR. Here, we find that HR induced by IR is drastically reduced when post-DNA damage replication is inhibited in mammalian cells. Both IR-induced RAD51 foci and HR events in the hprt gene are reduced in the presence of replication polymerase inhibitor aphidicolin (APH). Interestingly, we also detect reduced IR-induced toxicity in HR deficient cells when inhibiting post-DNA damage replication. When studying DSB formation following IR exposure, we find that apart from the direct DSBs the treatment also triggers formation of secondary DSBs peaking at 7-9 h after exposure. These secondary DSBs are restricted to newly replicated DNA and abolished by inhibiting post-DNA damage replication. Further, we find that IR-induced RAD51 foci are decreased by APH only in cells replicating at the time of IR exposure, suggesting distinct differences between IR-induced HR in S- and G2-phases of the cell cycle. Altogether, our data indicate that secondary replication-associated DSBs formed following exposure to IR are major substrates for IR-induced HR repair.","is_dataset_classified":null,"base_score":4.31748811353631,"endowment":4.31748811353631,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22505579","pmcid":"PMC3413124","openalex_id":"https://openalex.org/W2115366738","authors":[],"funders":[],"total_grants":0,"fwci":3.56,"citation_percentile":0.93928133,"influential_citations":0,"citation_trend":[{"year":2012,"count":3},{"year":2013,"count":9},{"year":2014,"count":7},{"year":2015,"count":10},{"year":2016,"count":6},{"year":2017,"count":6},{"year":2018,"count":3},{"year":2019,"count":3},{"year":2020,"count":5},{"year":2021,"count":5},{"year":2022,"count":5},{"year":2023,"count":6},{"year":2024,"count":3},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by-nc","oa_locations":[{"url":"https://academic.oup.com/nar/article-pdf/40/14/6585/16964895/gks315.pdf","host_type":"journal"},{"url":"https://academic.oup.com/nar/article-pdf/40/14/6585/16964895/gks315.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/nar/article-pdf/40/14/6585/16964895/gks315.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/nar/gks315","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22505579","host_type":"repository"},{"url":"https://idus.us.es/handle/11441/55888","host_type":"repository"},{"url":"http://eprints.whiterose.ac.uk/159259/1/Homologous%20recombination%20repairs%20secondary%20replication%20induced%20DNA%20double-strand%20breaks%20after%20ionizing%20radiation.pdf","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3413124","host_type":"repository"},{"url":"http://hdl.handle.net/11441/55888","host_type":"repository"},{"url":"http://nar.oxfordjournals.org/cgi/content/short/40/14/6585","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC3413124","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3413124?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["DNA Repair Mechanisms","Carcinogens and Genotoxicity Assessment","Cancer therapeutics and mechanisms","Animals","CHO Cells","Cell Line","Cricetinae","Cricetulus","DNA Breaks, Double-Stranded","DNA Replication","Humans","Radiation, Ionizing","Recombinational DNA Repair"],"mesh_terms":["Animals","Cell Line","Cricetulus","DNA Replication","Cricetinae","Humans","Radiation, Ionizing","CHO Cells","DNA Breaks, Double-Stranded","Recombinational DNA Repair"],"keywords":["RAD51","Aphidicolin","Biology","Homologous recombination","DNA replication","DNA","Molecular biology","Non-homologous end joining","DNA damage","DNA repair","Replication protein A","Homologous chromosome","Replication factor C","Gene","Cell biology","Control of chromosome duplication","Genetics","DNA-binding protein"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T13:41:09.209436Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}