{"doi":"10.1093/nar/gks059","title":"Functional and structural characterization of the mammalian TREX-2 complex that links transcription with nuclear messenger RNA export","abstract":null,"journal":"Nucleic Acids Research","year":2012,"id":653578,"datarank":0.7495818410646173,"base_score":4.997212273764115,"endowment":4.997212273764115,"self_citation_contribution":0.7495818410646173,"citation_network_contribution":0.0,"self_endowment_contribution":0.7495818410646173,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":147,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":562985,"name":"Sheila Lutz","orcid":"0000-0002-6729-4598","position":1,"is_corresponding":false},{"id":179948,"name":"Ed Hurt","orcid":null,"position":2,"is_corresponding":false},{"id":1705328,"name":"Ronald A. Laskey","orcid":null,"position":3,"is_corresponding":false},{"id":433629,"name":"Murray Stewart","orcid":"0000-0001-7925-7180","position":4,"is_corresponding":false},{"id":625894,"name":"Vihandha O. Wickramasinghe","orcid":"0000-0002-9438-3457","position":5,"is_corresponding":false},{"id":1705327,"name":"Divyang Jani","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Functional and structural characterization of the mammalian TREX-2 complex that links transcription with nuclear messenger RNA export","abstract":"Export of messenger RNA (mRNA) from the nucleus to the cytoplasm is a critical step in the gene expression pathway of eukaryotic cells. Here, we report the functional and structural characterization of the mammalian TREX-2 complex and show how it links transcription/processing with nuclear mRNA export. Mammalian TREX-2 is based on a germinal-centre associated nuclear protein (GANP) scaffold to which ENY2, PCID2 and centrins bind and depletion of any of these components inhibits mRNA export. The crystal structure of the GANP:ENY2 complex shows that two ENY2 chains interact directly with GANP, but they have different orientations from those observed on yeast Sac3. GANP is required to recruit ENY2 to nuclear pore complexes (NPCs), but ENY2 is not necessary to recruit GANP, which requires both its CID and MCM3AP domains, together with nucleoporin Nup153. GANP and ENY2 associate with RNA polymerase II and inhibition of mRNA processing redistributes GANP from NPCs into nuclear foci indicating that mammalian TREX-2 is associated with transcription. Thus, we implicate TREX-2 as an integral component of the mammalian mRNA export machinery where it links transcription and nuclear export by facilitating the transfer of mature mRNPs from the nuclear interior to NPCs.","is_dataset_classified":null,"base_score":4.997212273764115,"endowment":4.997212273764115,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22307388","pmcid":"PMC3378895","openalex_id":"https://openalex.org/W2016275804","authors":[],"funders":[{"funder_name":"Medical Research Council","grant_id":"MC_U105178939","title":null},{"funder_name":"Medical Research Council","grant_id":"U105178939","title":null},{"funder_name":"Wellcome Trust","grant_id":"unidentified","title":"unidentified"},{"funder_name":"Cancer Research UK","grant_id":"","title":null},{"funder_name":"Wellcome Trust","grant_id":"","title":null},{"funder_name":"Wellcome Trust","grant_id":"","title":null},{"funder_name":"Cancer Research UK","grant_id":"","title":null}],"total_grants":7,"fwci":4.4236,"citation_percentile":0.95588305,"influential_citations":0,"citation_trend":[{"year":2012,"count":3},{"year":2013,"count":11},{"year":2014,"count":12},{"year":2015,"count":10},{"year":2016,"count":11},{"year":2017,"count":5},{"year":2018,"count":11},{"year":2019,"count":11},{"year":2020,"count":8},{"year":2021,"count":10},{"year":2022,"count":7},{"year":2023,"count":11},{"year":2024,"count":15},{"year":2025,"count":14},{"year":2026,"count":7}],"oa_status":"gold","license":"cc-by-nc","oa_locations":[{"url":"https://academic.oup.com/nar/article-pdf/40/10/4562/16961443/gks059.pdf","host_type":"journal"},{"url":"https://academic.oup.com/nar/article-pdf/40/10/4562/16961443/gks059.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/nar/article-pdf/40/10/4562/16961443/gks059.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/nar/gks059","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22307388","host_type":"repository"},{"url":"http://hdl.handle.net/11343/257876","host_type":"repository"},{"url":"http://nar.oxfordjournals.org/cgi/content/short/40/10/4562","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3378895","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC3378895","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3378895?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1093/nar/gks059","host_type":""},{"url":"https://dx.doi.org/10.1093/nar/gks059","host_type":""},{"url":"https://doi.org/https://doi.org/10.1093/nar/gks059","host_type":""}],"fields_of_study":["RNA Research and Splicing","Nuclear Structure and Function","RNA regulation and disease","0301 basic medicine","0303 health sciences","03 medical and health sciences","Acetyltransferases","Active Transport, Cell Nucleus","Amino Acid Sequence","Cell Nucleus","Crystallography, X-Ray","Exodeoxyribonucleases","Humans","Intracellular Signaling Peptides and Proteins","Models, Molecular","Molecular Sequence Data","Nuclear Pore Complex Proteins","Nuclear Proteins","Phosphoproteins","RNA Transport","RNA, Messenger","Transcription Factors","Transcription, Genetic"],"mesh_terms":["Acetyltransferases","Amino Acid Sequence","Cell Nucleus","Exodeoxyribonucleases","Humans","Models, Molecular","Molecular Sequence Data","Nuclear Proteins","Phosphoproteins","RNA, Messenger","Transcription Factors","Transcription, Genetic","Crystallography, X-Ray","Active Transport, Cell Nucleus","Nuclear Pore Complex Proteins","RNA Transport","Intracellular Signaling Peptides and Proteins"],"keywords":["Nuclear pore","Nuclear export signal","Biology","Nucleoporin","Messenger RNA","Cell biology","Transcription (linguistics)","Nuclear transport","RNA","RNA polymerase II","RNA-binding protein","Cell nucleus","Transcription factor","Nuclear protein","Cytoplasm","Gene expression","Genetics","Gene","Promoter","Models, Molecular","570","Transcription, Genetic","Molecular Sequence Data","Active Transport, Cell Nucleus","Intracellular Signaling Peptides and Proteins","500","Nuclear Proteins","Crystallography, X-Ray","Phosphoproteins","RNA Transport","Nuclear Pore Complex Proteins","Exodeoxyribonucleases","Acetyltransferases","Humans","Amino Acid Sequence","RNA, Messenger","Molecular Biology","Transcription 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