{"doi":"10.1093/nar/gkq079","title":"Photomodulating RNA cleavage using photolabile circular antisense oligodeoxynucleotides","abstract":null,"journal":"Nucleic Acids Research","year":2010,"id":654312,"datarank":0.6038027536102726,"base_score":4.02535169073515,"endowment":4.02535169073515,"self_citation_contribution":0.6038027536102726,"citation_network_contribution":0.0,"self_endowment_contribution":0.6038027536102726,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":55,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1013657,"name":"Meng Su","orcid":"0000-0001-8485-2590","position":1,"is_corresponding":false},{"id":1707622,"name":"LiLi Yu","orcid":null,"position":2,"is_corresponding":false},{"id":308683,"name":"Cong Lv","orcid":"0009-0007-3225-3378","position":3,"is_corresponding":false},{"id":611154,"name":"Jie Wang","orcid":"0000-0003-3278-2708","position":4,"is_corresponding":false},{"id":1707623,"name":"ZhongJin Li","orcid":null,"position":5,"is_corresponding":false},{"id":1707621,"name":"XinJing Tang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Photomodulating RNA cleavage using photolabile circular antisense oligodeoxynucleotides","abstract":"Caged antisense oligodeoxynucleotides (asODNs) are synthesized by linking two ends of linear oligodeoxynucleotides using a photocleavable linker. Two of them (H30 and H40) have hairpin-like structures which show a large difference in thermal stability (Delta T(m) = 17.5 degrees C and 11.6 degrees C) comparing to uncaged ones. The other three (C20, C30 and C40) without stable secondary structures have the middle 20 deoxynucleotides complementary to 40-mer RNA. All caged asODNs have restricted opening which provides control over RNA/asODN interaction. RNase H assay results showed that 40-mer RNA digestion could be photo-modulated 2- to 3-fold upon light-activation with H30, H40, C30 and C40, while with C20, RNA digestion was almost not detectable; however, photo-activation triggered >20-fold increase of RNA digestion. And gel shift assays showed that it needed >0.04 microM H40 and 0.5 microM H30 to completely bind 0.02 microM 40-mer RNA, and for C40 and C30, it needed >0.2 microM and 0.5 microM for 0.02 microM 40-mer RNA binding. However, even 4 microM C20 was not able to fully bind the same concentration of 40-mer RNA. By simple adjustment of ring size of caged asODNs, we could successfully photoregulate their hybridization with mRNA and target RNA hydrolysis by RNase H with light activation.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20164090","pmcid":"PMC2887953","openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"gold","license":"cc-by-nc","oa_locations":[{"url":"https://academic.oup.com/nar/article-pdf/38/11/3848/16764381/gkq079.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/nar/article-pdf/38/11/3848/16764381/gkq079.pdf","host_type":"publisher"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2887953","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC2887953","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC2887953?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["DNA, Circular","Nucleic Acid Hybridization","Oligodeoxyribonucleotides, Antisense","RNA","Ribonuclease H","Ultraviolet Rays"],"mesh_terms":["Oligodeoxyribonucleotides, Antisense","DNA, Circular","RNA","Nucleic Acid Hybridization","Ultraviolet Rays","Ribonuclease H"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-11T05:46:56.776250Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}