{"doi":"10.1093/nar/gkp1154","title":"Definition of the interacting interfaces of Apobec3G and HIV-1 Vif using MAPPIT mutagenesis analysis","abstract":null,"journal":"Nucleic Acids Research","year":2010,"id":594876,"datarank":0.5926865577872142,"base_score":3.9512437185814275,"endowment":3.9512437185814275,"self_citation_contribution":0.5926865577872142,"citation_network_contribution":0.0,"self_endowment_contribution":0.5926865577872142,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":51,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1050976,"name":"Frank Peelman","orcid":"0000-0001-6852-8731","position":1,"is_corresponding":false},{"id":1523052,"name":"José Van der Heyden","orcid":null,"position":2,"is_corresponding":false},{"id":1523053,"name":"Isabel Uyttendaele","orcid":null,"position":3,"is_corresponding":false},{"id":1523054,"name":"Dominiek Catteeuw","orcid":null,"position":4,"is_corresponding":false},{"id":1523055,"name":"Annick Verhee","orcid":null,"position":5,"is_corresponding":false},{"id":1523056,"name":"Bertrand Van Schoubroeck","orcid":null,"position":6,"is_corresponding":false},{"id":1523057,"name":"Julia Kurth","orcid":null,"position":7,"is_corresponding":false},{"id":1523058,"name":"Sabine Hallenberger","orcid":null,"position":8,"is_corresponding":false},{"id":1523059,"name":"Reginald Clayton","orcid":null,"position":9,"is_corresponding":false},{"id":643017,"name":"Jan Tavernier","orcid":"0000-0002-7609-6462","position":10,"is_corresponding":false},{"id":1523051,"name":"Delphine Lavens","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Definition of the interacting interfaces of Apobec3G and HIV-1 Vif using MAPPIT mutagenesis analysis","abstract":"The host restriction factor Apobec3G is a cytidine deaminase that incorporates into HIV-1 virions and interferes with viral replication. The HIV-1 accessory protein Vif subverts Apobec3G by targeting it for proteasomal degradation. We propose a model in which Apobec3G N-terminal domains symmetrically interact via a head-to-head interface containing residues 122 RLYYFW 127. To validate this model and to characterize the Apobec3G-Apobec3G and the Apobec3G-Vif interactions, the mammalian protein-protein interaction trap two-hybrid technique was used. Mutations in the head-to-head interface abrogate the Apobec3G-Apobec3G interaction. All mutations that inhibit Apobec3G-Apobec3G binding also inhibit the Apobec3G-Vif interaction, indicating that the head-to head interface plays an important role in the interaction with Vif. Only the D128K, P129A and T32Q mutations specifically affect the Apobec3G-Vif association. In our model, D128, P129 and T32 cluster at the edge of the head-to-head interface, possibly forming a Vif binding site composed of two Apobec3G molecules. We propose that Vif either binds at the Apobec3G head-to-head interface or associates with an RNA-stabilized Apobec3G oligomer.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20015971","pmcid":"PMC2847223","openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"gold","license":"cc-by-nc","oa_locations":[{"url":"https://academic.oup.com/nar/article-pdf/38/6/1902/16770162/gkp1154.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/nar/article-pdf/38/6/1902/16770162/gkp1154.pdf","host_type":"publisher"},{"url":"https://biblio.ugent.be/publication/943512","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2847223","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC2847223","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC2847223?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":[],"mesh_terms":["Cell Line","Humans","Cytidine Deaminase","Cytosine Deaminase","Two-Hybrid System Techniques","Mutagenesis, Site-Directed","Binding Sites","Structural Homology, Protein","Dimerization","Models, Molecular","vif Gene Products, Human Immunodeficiency Virus","Protein Interaction Domains and Motifs","APOBEC-3G Deaminase"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-27T15:47:13.093571Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}