{"doi":"10.1093/nar/gkl300","title":"Kinetics of error generation in homologous B-family DNA polymerases","abstract":null,"journal":"Nucleic Acids Research","year":2006,"id":688216,"datarank":0.47670807455219194,"base_score":3.1780538303479458,"endowment":3.1780538303479458,"self_citation_contribution":0.47670807455219194,"citation_network_contribution":0.0,"self_endowment_contribution":0.47670807455219194,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":23,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":47431,"name":"W. Cooper","orcid":null,"position":1,"is_corresponding":false},{"id":1797894,"name":"L. Reha-Krantz","orcid":null,"position":2,"is_corresponding":false},{"id":1797895,"name":"S. S. Wallace","orcid":null,"position":3,"is_corresponding":false},{"id":1797893,"name":"M. Hogg","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Kinetics of error generation in homologous B-family DNA polymerases","abstract":"The kinetics of forming a proper Watson-Crick base pair as well incorporating bases opposite furan, an abasic site analog, have been well characterized for the B Family replicative DNA polymerase from bacteriophage T4. Structural studies of these reactions, however, have only been performed with the homologous enzyme from bacteriophage RB69. In this work, the homologous enzymes from RB69 and T4 were compared in parallel reactions to determine the relative abilities of the two polymerases to incorporate correct nucleotides as well as to form improper pairings. The kinetic rates for three different exonuclease mutants for each enzyme were measured for incorporation of an A opposite T and an A opposite furan as well as for the formation of A:C and T:T mismatches. The T4 exonuclease mutants were all approximately 2- to 7-fold more efficient than the corresponding RB69 exonuclease mutants depending on whether a T or furan was in the templating position and which exonuclease mutant was used. The rates for mismatch formation by T4 were significantly reduced compared with incorporation opposite furan, much more so than the corresponding RB69 mutant. These results show that there are kinetic differences between the two enzymes but they are not large enough to preclude structural assumptions for T4 DNA polymerase based on the known structure of the RB69 DNA polymerase.","is_dataset_classified":null,"base_score":3.1780538303479458,"endowment":3.1780538303479458,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16687658","pmcid":"PMC1459414","openalex_id":"https://openalex.org/W2137463654","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"R01 CA052040","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA52040","title":null},{"funder_name":"Natural Sciences and Engineering Research Council of Canada","grant_id":"unidentified","title":"unidentified"},{"funder_name":"National Institutes of Health","grant_id":"2R01CA052040-19A1","title":"Processing of Free Radical Radiation Damage by Human DNA Polymerases"}],"total_grants":4,"fwci":1.0174,"citation_percentile":0.74028154,"influential_citations":2,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":2},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2023,"count":1},{"year":2024,"count":1}],"oa_status":"gold","license":"other-oa","oa_locations":[{"url":"https://academic.oup.com/nar/article-pdf/34/9/2528/7129448/gkl300.pdf","host_type":"journal"},{"url":"https://doi.org/10.1093/nar/gkl300","host_type":"GOLD"},{"url":"https://academic.oup.com/nar/article-pdf/34/9/2528/7129448/gkl300.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/nar/article-pdf/34/9/2528/7129448/gkl300.pdf","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16687658","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1459414","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.276.5335","host_type":""},{"url":"https://europepmc.org/articles/PMC1459414","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC1459414?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1093/nar/gkl300","host_type":""},{"url":"https://dx.doi.org/10.1093/nar/gkl300","host_type":""}],"fields_of_study":["DNA Repair Mechanisms","DNA and Nucleic Acid Chemistry","Bacterial Genetics and Biotechnology","Biology","Medicine","0301 basic medicine","0303 health sciences","03 medical and health sciences","Base Pair Mismatch","Base Pairing","Binding Sites","DNA","DNA-Directed DNA Polymerase","Deoxyadenine Nucleotides","Furans","Kinetics","Mutation","Thymine","Viral Proteins"],"mesh_terms":["Binding Sites","Deoxyadenine Nucleotides","DNA","DNA-Directed DNA Polymerase","Furans","Kinetics","Mutation","Thymine","Viral Proteins","Base Pairing","Base Pair Mismatch"],"keywords":["Exonuclease","DNA polymerase","Biology","Polymerase","DNA","Base pair","Mutant","Biochemistry","Genetics","Molecular biology","Gene","Binding Sites","Base Pair Mismatch","DNA-Directed DNA Polymerase","Article","Kinetics","Viral Proteins","Deoxyadenine Nucleotides","Mutation","Furans","Base Pairing","Thymine"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-19T14:07:19.957385Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}