{"doi":"10.1093/nar/gki741","title":"Unfolding of DNA quadruplexes induced by HIV-1 nucleocapsid protein","abstract":null,"journal":"Nucleic Acids Research","year":2005,"id":645851,"datarank":0.6190701577567639,"base_score":4.127134385045092,"endowment":4.127134385045092,"self_citation_contribution":0.6190701577567639,"citation_network_contribution":0.0,"self_endowment_contribution":0.6190701577567639,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":61,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1681870,"name":"B. I. Kankia","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Unfolding of DNA quadruplexes induced by HIV-1 nucleocapsid protein","abstract":"The human immunodeficiency virus type 1 nucleocapsid protein (NC) is a nucleic acid chaperone that catalyzes the rearrangement of nucleic acids into their thermodynamically most stable structures. In the present study, a combination of optical and thermodynamic techniques were used to characterize the influence of NC on the secondary structure, thermal stability and energetics of monomolecular DNA quadruplexes formed by the sequence d(GGTTGGTGTGGTTGG) in the presence of K+ or Sr2+. Circular dichroism studies demonstrate that NC effectively unfolds the quadruplexes. Studies carried out with NC variants suggest that destabilization is mediated by the zinc fingers of NC. Calorimetric studies reveal that NC destabilization is enthalpic in origin, probably owing to unstacking of the G-quartets upon protein binding. In contrast, parallel studies performed on a related DNA duplex reveal that under conditions where NC readily destabilizes and unfolds the quadruplexes, its effect on the DNA duplex is much less pronounced. The differences in NC's ability to destabilize quadruplex versus duplex is in accordance with the higher DeltaG of melting for the latter, and with the inverse correlation between nucleic acid stability and the destabilizing activity of NC.","is_dataset_classified":null,"base_score":4.127134385045092,"endowment":4.127134385045092,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16077025","pmcid":"PMC1182697","openalex_id":"https://openalex.org/W2124087662","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM065056","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM65056","title":null}],"total_grants":2,"fwci":2.3861,"citation_percentile":0.88501363,"influential_citations":0,"citation_trend":[{"year":2012,"count":5},{"year":2013,"count":5},{"year":2014,"count":4},{"year":2015,"count":1},{"year":2016,"count":1},{"year":2017,"count":2},{"year":2018,"count":2},{"year":2019,"count":2},{"year":2022,"count":2},{"year":2023,"count":2},{"year":2025,"count":3}],"oa_status":"gold","license":null,"oa_locations":[{"url":"https://academic.oup.com/nar/article-pdf/33/14/4395/6154077/gki741.pdf","host_type":"journal"},{"url":"https://academic.oup.com/nar/article-pdf/33/14/4395/6154077/gki741.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/nar/article-pdf/33/14/4395/6154077/gki741.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/nar/gki741","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16077025","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.281.7484","host_type":""},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.566.871","host_type":""},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.585.5781","host_type":""},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.585.750","host_type":""},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1182697","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC1182697","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC1182697?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["DNA and Nucleic Acid Chemistry","Advanced biosensing and bioanalysis techniques","RNA Interference and Gene Delivery","Aptamers, Nucleotide","Base Sequence","Calorimetry","Circular Dichroism","DNA","G-Quadruplexes","HIV-1","Molecular Chaperones","Molecular Sequence Data","Nucleic Acid Conformation","Nucleic Acid Denaturation","Nucleocapsid Proteins","Oligonucleotides","Potassium","Salts","Spectrophotometry, Ultraviolet","Strontium","Temperature"],"mesh_terms":["Base Sequence","Calorimetry","Circular Dichroism","DNA","Molecular Sequence Data","Nucleic Acid Conformation","Nucleic Acid Denaturation","Oligonucleotides","Potassium","Salts","Spectrophotometry, Ultraviolet","Strontium","Temperature","HIV-1","Molecular Chaperones","Nucleocapsid Proteins","Aptamers, Nucleotide","G-Quadruplexes"],"keywords":["Nucleic acid","Duplex (building)","DNA","Biology","Circular dichroism","G-quadruplex","Nucleic Acid Denaturation","Biophysics","Chemical stability","Protein secondary structure","Nucleic acid structure","Nucleic acid thermodynamics","Crystallography","Biochemistry","RNA","Base sequence","Chemistry"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"refseq"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-09T10:22:59.391796Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}