{"doi":"10.1093/nar/gkaf1080","title":"Chromatin context shapes DNA damage formation and nucleotide excision repair dynamics in <i>Caenorhabditis elegans</i>","abstract":"DNA damage formation and repair are influenced by the genomic landscape, yet how chromatin and transcriptional activity shape these processes at a whole-organism scale remains incompletely understood. Using Caenorhabditis elegans, a widely used model organism to study DNA repair and related processes, we present comprehensive, time-course maps of ultraviolet-induced DNA damage and excision repair, revealing how chromatin context and transcription dictate the spatiotemporal patterns of damage and repair. Of the two repair pathways-global repair and transcription-coupled repair-global repair predominates, removing the majority of the lesions; and notably, (6-4) photoproducts are removed by transcription-coupled repair at an extent comparable to cyclobutane pyrimidine dimers, a feature not previously observed in animals. Integration of damage and repair profiles with chromatin features reveals that, despite non-uniform damage formation, repair efficiency is the primary determinant of residual damage. Finally, repair around accessible regions exhibit nucleosome-size periodicity, reflecting underlying nucleosome architecture. Together, these findings establish C. elegans as a valuable model organism for interrogating damage formation and repair within a chromatin context and reveal species-specific features that broaden our understanding of DNA repair mechanisms across metazoans.","journal":"Nucleic Acids Research","year":2025,"id":580136,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8748,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1490960,"name":"Cem Azgari","orcid":"0000-0001-7029-6298","position":1,"is_corresponding":false},{"id":342364,"name":"Laura A. Lindsey‐Boltz","orcid":"0000-0001-6493-8194","position":2,"is_corresponding":false},{"id":564412,"name":"Ogün Adebalı","orcid":"0000-0001-9213-4070","position":3,"is_corresponding":false},{"id":237019,"name":"Aziz Sancar","orcid":"0000-0001-6469-4900","position":4,"is_corresponding":false},{"id":1106866,"name":"Cansu Köse","orcid":"0000-0002-2024-3566","position":0,"is_corresponding":true}],"reference_count":81,"raw_metadata":null,"created_at":"2026-07-19T02:58:38.868285Z","pmid":"41189058","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}