{"doi":"10.1093/nar/gkae910","title":"Structural and functional insights into the interaction between the bacteriophage T4 DNA processing proteins gp32 and Dda","abstract":"Bacteriophage T4 is a classic model system for studying the mechanisms of DNA processing. A key protein in T4 DNA processing is the gp32 single-stranded DNA-binding protein. gp32 has two key functions: it binds cooperatively to single-stranded DNA (ssDNA) to protect it from nucleases and remove regions of secondary structure, and it recruits proteins to initiate DNA processes including replication and repair. Dda is a T4 helicase recruited by gp32, and we purified and crystallized a gp32-Dda-ssDNA complex. The low-resolution structure revealed how the C-terminus of gp32 engages Dda. Analytical ultracentrifugation analyses were consistent with the crystal structure. An optimal Dda binding peptide from the gp32 C-terminus was identified using surface plasmon resonance. The crystal structure of the Dda-peptide complex was consistent with the corresponding interaction in the gp32-Dda-ssDNA structure. A Dda-dependent DNA unwinding assay supported the structural conclusions and confirmed that the bound gp32 sequesters the ssDNA generated by Dda. The structure of the gp32-Dda-ssDNA complex, together with the known structure of the gp32 body, reveals the entire ssDNA binding surface of gp32. gp32-Dda-ssDNA complexes in the crystal are connected by the N-terminal region of one gp32 binding to an adjacent gp32, and this provides key insights into this interaction.","journal":"Nucleic Acids Research","year":2024,"id":439210,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9562,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":381377,"name":"Mi‐Kyung Yun","orcid":"0000-0003-1213-5714","position":1,"is_corresponding":false},{"id":381381,"name":"Zhenmei Li","orcid":"0000-0001-5229-3798","position":2,"is_corresponding":false},{"id":464862,"name":"M. Brett Waddell","orcid":null,"position":3,"is_corresponding":false},{"id":556040,"name":"Amanda Nourse","orcid":"0000-0003-4595-5321","position":4,"is_corresponding":false},{"id":1229614,"name":"Kelly Churion","orcid":"0000-0002-4346-7562","position":5,"is_corresponding":false},{"id":1250462,"name":"Kenneth N. Kreuzer","orcid":null,"position":6,"is_corresponding":false},{"id":304981,"name":"Alicia K. Byrd","orcid":"0000-0001-5484-0759","position":7,"is_corresponding":false},{"id":381385,"name":"Stephen W. White","orcid":"0000-0001-8188-5944","position":8,"is_corresponding":false},{"id":1249824,"name":"Xiaoping He","orcid":"0000-0002-0697-6501","position":0,"is_corresponding":true}],"reference_count":78,"raw_metadata":null,"created_at":"2026-07-19T02:00:47.992543Z","pmid":"39417586","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}