{"doi":"10.1093/nar/gkae574","title":"Nuclear F-actin assembly on damaged chromatin is regulated by DYRK1A and Spir1 phosphorylation","abstract":"Nuclear actin-based movements support DNA double-strand break (DSB) repair. However, molecular determinants that promote filamentous actin (F-actin) formation on the damaged chromatin remain undefined. Here we describe the DYRK1A kinase as a nuclear activity that promotes local F-actin assembly to support DSB mobility and repair, accomplished in part by its targeting of actin nucleator spire homolog 1 (Spir1). Indeed, perturbing DYRK1A-dependent phosphorylation of S482 mis-regulated Spir1 accumulation at damaged-modified chromatin, and led to compromised DSB-associated actin polymerization and attenuated DNA repair. Our findings uncover a role of the DYRK1A-Spir1 axis in nuclear actin dynamics during early DSB responses, and highlight the intricate details of nuclear cytoskeletal network in DSB repair and genome stability maintenance.","journal":"Nucleic Acids Research","year":2024,"id":447062,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9555,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1265204,"name":"Nan Xiong","orcid":null,"position":1,"is_corresponding":false},{"id":462078,"name":"Kirk L. West","orcid":"0000-0002-9894-4549","position":2,"is_corresponding":false},{"id":1265205,"name":"Manton Man‐hon Leung","orcid":null,"position":3,"is_corresponding":false},{"id":1265206,"name":"Yick Pang Ching","orcid":null,"position":4,"is_corresponding":false},{"id":1264686,"name":"Jun Huang","orcid":"0000-0003-4615-4411","position":5,"is_corresponding":false},{"id":667772,"name":"Jian Yuan","orcid":"0000-0002-2801-8849","position":6,"is_corresponding":false},{"id":1264687,"name":"Cheng‐han Yu","orcid":"0000-0002-8821-8877","position":7,"is_corresponding":false},{"id":385347,"name":"Justin Leung","orcid":"0000-0003-4601-390X","position":8,"is_corresponding":false},{"id":1264688,"name":"Michael S.Y. Huen","orcid":"0000-0003-1774-2425","position":9,"is_corresponding":false},{"id":1264685,"name":"Junshi Li","orcid":"0009-0008-3929-9616","position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":null,"created_at":"2026-07-19T02:01:59.101070Z","pmid":"38966995","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}