{"doi":"10.1093/nar/gkad310","title":"RecF protein targeting to post-replication (daughter strand) gaps II: RecF interaction with replisomes","abstract":"The bacterial RecF, RecO, and RecR proteins are an epistasis group involved in loading RecA protein into post-replication gaps. However, the targeting mechanism that brings these proteins to appropriate gaps is unclear. Here, we propose that targeting may involve a direct interaction between RecF and DnaN. In vivo, RecF is commonly found at the replication fork. Over-expression of RecF, but not RecO or a RecF ATPase mutant, is extremely toxic to cells. We provide evidence that the molecular basis of the toxicity lies in replisome destabilization. RecF over-expression leads to loss of genomic replisomes, increased recombination associated with post-replication gaps, increased plasmid loss, and SOS induction. Using three different methods, we document direct interactions of RecF with the DnaN β-clamp and DnaG primase that may underlie the replisome effects. In a single-molecule rolling-circle replication system in vitro, physiological levels of RecF protein trigger post-replication gap formation. We suggest that the RecF interactions, particularly with DnaN, reflect a functional link between post-replication gap creation and gap processing by RecA. RecF's varied interactions may begin to explain how the RecFOR system is targeted to rare lesion-containing post-replication gaps, avoiding the potentially deleterious RecA loading onto thousands of other gaps created during replication.","journal":"Nucleic Acids Research","year":2023,"id":333242,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":23,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9549,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":972020,"name":"Gurleen Kaur","orcid":"0000-0003-4466-2279","position":1,"is_corresponding":false},{"id":406652,"name":"Megan E Cherry","orcid":"0000-0003-0874-2853","position":2,"is_corresponding":false},{"id":406644,"name":"Sarah S. Henrikus","orcid":"0000-0002-4873-931X","position":3,"is_corresponding":false},{"id":1060501,"name":"Nina J. Bonde","orcid":"0000-0001-8297-165X","position":4,"is_corresponding":false},{"id":1060502,"name":"Nischal Sharma","orcid":"0000-0002-7670-5571","position":5,"is_corresponding":false},{"id":1061073,"name":"Hope A. Beyer","orcid":null,"position":6,"is_corresponding":false},{"id":406653,"name":"Elizabeth A. Wood","orcid":"0000-0002-7769-8481","position":7,"is_corresponding":false},{"id":700036,"name":"Sindhu Chitteni‐Pattu","orcid":null,"position":8,"is_corresponding":false},{"id":406657,"name":"Antoine M. van Oijen","orcid":"0000-0002-1794-5161","position":9,"is_corresponding":false},{"id":271609,"name":"Andrew Robinson","orcid":"0000-0002-3544-0976","position":10,"is_corresponding":false},{"id":406656,"name":"Michael M. Cox","orcid":"0000-0003-3606-5722","position":11,"is_corresponding":false},{"id":406645,"name":"Camille Henry","orcid":"0000-0002-4011-6767","position":0,"is_corresponding":true}],"reference_count":131,"raw_metadata":null,"created_at":"2026-07-19T01:09:39.719497Z","pmid":"37125644","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}