{"doi":"10.1093/nar/gkad286","title":"Dynamics of release factor recycling during translation termination in bacteria","abstract":"In bacteria, release of newly synthesized proteins from ribosomes during translation termination is catalyzed by class-I release factors (RFs) RF1 or RF2, reading UAA and UAG or UAA and UGA codons, respectively. Class-I RFs are recycled from the post-termination ribosome by a class-II RF, the GTPase RF3, which accelerates ribosome intersubunit rotation and class-I RF dissociation. How conformational states of the ribosome are coupled to the binding and dissociation of the RFs remains unclear and the importance of ribosome-catalyzed guanine nucleotide exchange on RF3 for RF3 recycling in vivo has been disputed. Here, we profile these molecular events using a single-molecule fluorescence assay to clarify the timings of RF3 binding and ribosome intersubunit rotation that trigger class-I RF dissociation, GTP hydrolysis, and RF3 dissociation. These findings in conjunction with quantitative modeling of intracellular termination flows reveal rapid ribosome-dependent guanine nucleotide exchange to be crucial for RF3 action in vivo.","journal":"Nucleic Acids Research","year":2023,"id":384333,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9471,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1151558,"name":"Michael Y. Pavlov","orcid":"0000-0002-3794-1870","position":1,"is_corresponding":false},{"id":527443,"name":"Jingji Zhang","orcid":"0000-0003-4989-4597","position":2,"is_corresponding":false},{"id":750802,"name":"Gabriele Indrisiunaite","orcid":"0000-0003-0742-3906","position":3,"is_corresponding":false},{"id":463182,"name":"Jinfan Wang","orcid":"0000-0003-4170-9289","position":4,"is_corresponding":false},{"id":1151559,"name":"Michael R. Lawson","orcid":"0000-0003-3554-8423","position":5,"is_corresponding":false},{"id":750803,"name":"Måns Ehrenberg","orcid":"0000-0002-0552-882X","position":6,"is_corresponding":false},{"id":319748,"name":"Joseph D. Puglisi","orcid":"0000-0001-9268-5112","position":7,"is_corresponding":false},{"id":577464,"name":"Arjun Prabhakar","orcid":"0000-0002-5589-2558","position":0,"is_corresponding":true}],"reference_count":55,"raw_metadata":null,"created_at":"2026-07-19T01:17:33.041385Z","pmid":"37102635","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}