{"doi":"10.1093/nar/gkad194","title":"Cryo-EM reconstruction of the human 40S ribosomal subunit at 2.15 Å resolution","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>The chemical modification of ribosomal RNA and proteins is critical for ribosome assembly, for protein synthesis and may drive ribosome specialisation in development and disease. However, the inability to accurately visualise these modifications has limited mechanistic understanding of the role of these modifications in ribosome function. Here we report the 2.15 Å resolution cryo-EM reconstruction of the human 40S ribosomal subunit. We directly visualise post-transcriptional modifications within the 18S rRNA and four post-translational modifications of ribosomal proteins. Additionally, we interpret the solvation shells in the core regions of the 40S ribosomal subunit and reveal how potassium and magnesium ions establish both universally conserved and eukaryote-specific coordination to promote the stabilisation and folding of key ribosomal elements. This work provides unprecedented structural details for the human 40S ribosomal subunit that will serve as an important reference for unravelling the functional role of ribosomal RNA modifications.</jats:p>","journal":"Nucleic Acids Research","year":2023,"id":33305,"datarank":0.8444766265688988,"base_score":3.2188758248682006,"endowment":3.2188758248682006,"self_citation_contribution":0.48283137373023016,"citation_network_contribution":0.3616452528386686,"self_endowment_contribution":0.48283137373023016,"citer_contribution":0.3616452528386686,"corpus_percentile":null,"corpus_rank":null,"citation_count":24,"citer_count":24,"citers_with_citation_signal":20,"citers_with_endowment":20,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":174595,"name":"Kyle C Dent","orcid":null,"position":1,"is_corresponding":false},{"id":174596,"name":"Tobias Spikes","orcid":null,"position":2,"is_corresponding":false},{"id":174597,"name":"Alan J Warren","orcid":"0000-0001-9277-4553","position":3,"is_corresponding":false},{"id":174593,"name":"Simone Pellegrino","orcid":"0000-0001-6302-2774","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.2188758248682006,"endowment":3.2188758248682006,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36951107","pmcid":"PMC10164566","openalex_id":"https://openalex.org/W4353112023","authors":[],"funders":[{"funder_name":"UK Medical Research Council","grant_id":"MR/T012412/1","title":"Molecular mechanisms of eukaryotic ribosome assembly"},{"funder_name":"European Cooperation in Science and Technology","grant_id":"CA18233","title":null},{"funder_name":"European Network for Innovative Diagnosis and treatment of Chronic Neutropenias, INNOCHRON","grant_id":"CA21154","title":null},{"funder_name":"Cambridge National Institute for Health Research Biomedical Research Centre","grant_id":"BRC-1215-20014","title":null},{"funder_name":"Wellcome Trust","grant_id":"206171/Z/17/Z","title":null},{"funder_name":"Wellcome Trust","grant_id":"202905/Z/16/Z","title":null},{"funder_name":"Medical Research Council","grant_id":"MC_U105161083","title":"Molecular mechanisms of eukaryotic ribosome biogenesis"},{"funder_name":"Wellcome Trust","grant_id":"unidentified","title":"unidentified"},{"funder_name":"Wellcome Trust","grant_id":"202905","title":"An electron cryo-microscopy resource for macromolecular structure determination in the University of Cambridge"},{"funder_name":"Wellcome Trust","grant_id":"206171","title":"An advanced cryoEM instrument for the University of Cambridge"},{"funder_name":"Medical Research Council","grant_id":"","title":null},{"funder_name":"University of Cambridge","grant_id":"","title":null},{"funder_name":"Translational control in Cancer European Network, TRANSLACORE","grant_id":"","title":null},{"funder_name":"Departments of Biochemistry and Chemistry","grant_id":"","title":null},{"funder_name":"Kay Kendall Leukaemia Fund","grant_id":"","title":null},{"funder_name":"Schools of Biological Sciences and Clinical 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and protein synthesis mechanisms","RNA modifications and cancer","Advanced Electron Microscopy Techniques and Applications","Medicine","Biology","Chemistry","0301 basic medicine","0303 health sciences","03 medical and health sciences","Humans","Ribosome Subunits, Small, Eukaryotic","Cryoelectron Microscopy","Ribosomal Proteins","Ribosomes","RNA, Ribosomal","RNA, Ribosomal, 18S"],"mesh_terms":["Humans","Ribosomal Proteins","Ribosomes","RNA, Ribosomal","RNA, Ribosomal, 18S","Cryoelectron Microscopy","Ribosome Subunits, Small, Eukaryotic"],"keywords":["Biology","Eukaryotic Small Ribosomal Subunit","Ribosomal RNA","Protein subunit","Ribosomal DNA","Computational biology","Resolution (logic)","Molecular biology","Genetics","Ribosome","Phylogenetics","Gene","RNA","Artificial intelligence","Ribosome Subunits, Small, Eukaryotic","Ribosomal Proteins","Structural Biology","RNA, Ribosomal","Cryoelectron Microscopy","RNA, Ribosomal, 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