{"doi":"10.1093/nar/gkad1209","title":"BG4 antibody can recognize telomeric G-quadruplexes harboring destabilizing base modifications and lesions","abstract":"BG4 is a single-chain variable fragment antibody shown to bind various G-quadruplex (GQ) topologies with high affinity and specificity, and to detect GQ in cells, including GQ structures formed within telomeric TTAGGG repeats. Here, we used ELISA and single-molecule pull-down (SiMPull) detection to test how various lengths and GQ destabilizing base modifications in telomeric DNA constructs alter BG4 binding. We observed high-affinity BG4 binding to telomeric GQ independent of telomere length, although three telomeric repeat constructs that cannot form stable intramolecular GQ showed reduced affinity. A single guanine substitution with 8-aza-7-deaza-G, T, A, or C reduced affinity to varying degrees depending on the location and base type, whereas two G substitutions in the telomeric construct dramatically reduced or abolished binding. Substitution with damaged bases 8-oxoguanine and O6-methylguanine failed to prevent BG4 binding although affinity was reduced depending on lesion location. SiMPull combined with FRET revealed that BG4 binding promotes folding of telomeric GQ harboring a G to T substitution or 8-oxoguanine. Atomic force microscopy revealed that BG4 binds telomeric GQ with a 1:1 stoichiometry. Collectively, our data suggest that BG4 can recognize partially folded telomeric GQ structures and promote telomeric GQ stability.","journal":"Nucleic Acids Research","year":2023,"id":336749,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9637,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":475672,"name":"Tapas Paul","orcid":"0000-0001-7508-5676","position":1,"is_corresponding":false},{"id":323488,"name":"Samantha L. Sanford","orcid":"0000-0002-6517-3557","position":2,"is_corresponding":false},{"id":676201,"name":"Brittani L Schnable","orcid":null,"position":3,"is_corresponding":false},{"id":734767,"name":"Ariana C. Detwiler","orcid":"0000-0002-9311-5899","position":4,"is_corresponding":false},{"id":808442,"name":"Sanjana A. Thosar","orcid":null,"position":5,"is_corresponding":false},{"id":182885,"name":"Bennett Van Houten","orcid":"0000-0002-4009-2478","position":6,"is_corresponding":false},{"id":284455,"name":"Sua Myong","orcid":"0000-0001-9098-3423","position":7,"is_corresponding":false},{"id":323491,"name":"Patricia L. Opresko","orcid":"0000-0002-6470-2189","position":8,"is_corresponding":false},{"id":506061,"name":"Samuel A. Johnson","orcid":"0000-0002-0015-8866","position":0,"is_corresponding":true}],"reference_count":66,"raw_metadata":null,"created_at":"2026-07-19T01:10:21.947540Z","pmid":"38153143","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}