{"doi":"10.1093/nar/gkad049","title":"LINE-1 retrotransposon expression in cancerous, epithelial and neuronal cells revealed by 5′ single-cell RNA-Seq","abstract":"LINE-1 retrotransposons are sequences capable of copying themselves to new genomic loci via an RNA intermediate. New studies implicate LINE-1 in a range of diseases, especially in the context of aging, but without an accurate understanding of where and when LINE-1 is expressed, a full accounting of its role in health and disease is not possible. We therefore developed a method-5' scL1seq-that makes use of a widely available library preparation method (10x Genomics 5' single cell RNA-seq) to measure LINE-1 expression in tens of thousands of single cells. We recapitulated the known pattern of LINE-1 expression in tumors-present in cancer cells, absent from immune cells-and identified hitherto undescribed LINE-1 expression in human epithelial cells and mouse hippocampal neurons. In both cases, we saw a modest increase with age, supporting recent research connecting LINE-1 to age related diseases.","journal":"Nucleic Acids Research","year":2023,"id":331438,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":28,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9524,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":49810,"name":"Larisa Kagermazova","orcid":"0009-0004-7494-284X","position":1,"is_corresponding":false},{"id":418184,"name":"Nicole Doudican","orcid":"0000-0003-3827-9644","position":2,"is_corresponding":false},{"id":484040,"name":"Nicholas Frazzette","orcid":null,"position":3,"is_corresponding":false},{"id":323548,"name":"Efiyenia Ismini Kaparos","orcid":"0000-0003-2611-7648","position":4,"is_corresponding":false},{"id":15984,"name":"Shane A. Evans","orcid":null,"position":5,"is_corresponding":false},{"id":619371,"name":"Azucena Rocha","orcid":null,"position":6,"is_corresponding":false},{"id":98109,"name":"John M. Sedivy","orcid":"0000-0001-9363-4253","position":7,"is_corresponding":false},{"id":583341,"name":"Nicola Neretti","orcid":"0000-0002-9552-1663","position":8,"is_corresponding":false},{"id":401838,"name":"John A. Carucci","orcid":"0000-0001-6817-9439","position":9,"is_corresponding":false},{"id":49826,"name":"Jef D. Boeke","orcid":"0000-0001-5322-4946","position":10,"is_corresponding":false},{"id":2222,"name":"David Fenyö","orcid":"0000-0001-5049-3825","position":11,"is_corresponding":false},{"id":446821,"name":"Wilson McKerrow","orcid":"0000-0001-6328-853X","position":0,"is_corresponding":true}],"reference_count":83,"raw_metadata":null,"created_at":"2026-07-19T01:09:19.317824Z","pmid":"36744437","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}