{"doi":"10.1093/nar/gkac647","title":"Structural basis of DNA packaging by a ring-type ATPase from an archetypal viral system","abstract":"Many essential cellular processes rely on substrate rotation or translocation by a multi-subunit, ring-type NTPase. A large number of double-stranded DNA viruses, including tailed bacteriophages and herpes viruses, use a homomeric ring ATPase to processively translocate viral genomic DNA into procapsids during assembly. Our current understanding of viral DNA packaging comes from three archetypal bacteriophage systems: cos, pac and phi29. Detailed mechanistic understanding exists for pac and phi29, but not for cos. Here, we reconstituted in vitro a cos packaging system based on bacteriophage HK97 and provided a detailed biochemical and structural description. We used a photobleaching-based, single-molecule assay to determine the stoichiometry of the DNA-translocating ATPase large terminase. Crystal structures of the large terminase and DNA-recruiting small terminase, a first for a biochemically defined cos system, reveal mechanistic similarities between cos and pac systems. At the same time, mutational and biochemical analyses indicate a new regulatory mechanism for ATPase multimerization and coordination in the HK97 system. This work therefore establishes a framework for studying the evolutionary relationships between ATP-dependent DNA translocation machineries in double-stranded DNA viruses.","journal":"Nucleic Acids Research","year":2022,"id":261192,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9492,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":916024,"name":"Shelley Grimes","orcid":null,"position":1,"is_corresponding":false},{"id":500919,"name":"Alexis Huet","orcid":"0000-0001-6069-4460","position":2,"is_corresponding":false},{"id":431011,"name":"Robert L. Duda","orcid":"0000-0002-9517-2731","position":3,"is_corresponding":false},{"id":915445,"name":"Maria Chechik","orcid":"0000-0001-8413-4230","position":4,"is_corresponding":false},{"id":544230,"name":"Joseph Gault","orcid":"0000-0001-7686-2983","position":5,"is_corresponding":false},{"id":250509,"name":"Carol V. Robinson","orcid":"0000-0001-7829-5505","position":6,"is_corresponding":false},{"id":450262,"name":"Roger W. Hendrix","orcid":null,"position":7,"is_corresponding":false},{"id":371053,"name":"Paul J. Jardine","orcid":"0000-0002-1090-0550","position":8,"is_corresponding":false},{"id":349002,"name":"James F. Conway","orcid":"0000-0002-6581-4748","position":9,"is_corresponding":false},{"id":823378,"name":"Christoph G. Baumann","orcid":"0000-0002-8818-972X","position":10,"is_corresponding":false},{"id":106085,"name":"Alfred A. Antson","orcid":"0000-0002-4533-3816","position":11,"is_corresponding":false},{"id":915444,"name":"Herman K.H. Fung","orcid":"0000-0001-9568-1782","position":0,"is_corresponding":true}],"reference_count":84,"raw_metadata":null,"created_at":"2026-07-19T00:26:12.158801Z","pmid":"35947691","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}