{"doi":"10.1093/nar/gkab510","title":"Stabilization of Hfq-mediated translational repression by the co-repressor Crc in <i>Pseudomonas aeruginosa</i>","abstract":"In Pseudomonas aeruginosa the RNA chaperone Hfq and the catabolite repression control protein (Crc) govern translation of numerous transcripts during carbon catabolite repression. Here, Crc was shown to enhance Hfq-mediated translational repression of several mRNAs. We have developed a single-molecule fluorescence assay to quantitatively assess the cooperation of Hfq and Crc to form a repressive complex on a RNA, encompassing the translation initiation region and the proximal coding sequence of the P. aeruginosa amiE gene. The presence of Crc did not change the amiE RNA-Hfq interaction lifetimes, whereas it changed the equilibrium towards more stable repressive complexes. This observation is in accord with Cryo-EM analyses, which showed an increased compactness of the repressive Hfq/Crc/RNA assemblies. These biophysical studies revealed how Crc protein kinetically stabilizes Hfq/RNA complexes, and how the two proteins together fold a large segment of the mRNA into a more compact translationally repressive structure. In fact, the presence of Crc resulted in stronger translational repression in vitro and in a significantly reduced half-life of the target amiE mRNA in vivo. Although Hfq is well-known to act with small regulatory RNAs, this study shows how Hfq can collaborate with another protein to down-regulate translation of mRNAs that become targets for the degradative machinery.","journal":"Nucleic Acids Research","year":2021,"id":164954,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":37,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.959,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":688509,"name":"Flavia Bassani","orcid":null,"position":1,"is_corresponding":false},{"id":662109,"name":"Tom Dendooven","orcid":"0000-0002-9847-0910","position":2,"is_corresponding":false},{"id":643243,"name":"Elisabeth Sonnleitner","orcid":"0000-0002-6961-9536","position":3,"is_corresponding":false},{"id":688510,"name":"Marlena Rozner","orcid":null,"position":4,"is_corresponding":false},{"id":688511,"name":"Tanino G. Albanese","orcid":null,"position":5,"is_corresponding":false},{"id":136622,"name":"Armin Resch","orcid":null,"position":6,"is_corresponding":false},{"id":561596,"name":"Ben F. Luisi","orcid":"0000-0003-1144-9877","position":7,"is_corresponding":false},{"id":396831,"name":"Sarah A. Woodson","orcid":"0000-0003-0170-1987","position":8,"is_corresponding":false},{"id":643245,"name":"Udo Bläsi","orcid":"0000-0003-4830-257X","position":9,"is_corresponding":false},{"id":455004,"name":"Ewelina M. Małecka","orcid":"0000-0001-8482-7897","position":0,"is_corresponding":true}],"reference_count":56,"raw_metadata":null,"created_at":"2026-07-18T23:45:36.425208Z","pmid":"34139006","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}