{"doi":"10.1093/nar/gkaa340","title":"Dengue virus targets RBM10 deregulating host cell splicing and innate immune response","abstract":"RNA-seq experiments previously performed by our laboratories showed enrichment in intronic sequences and alterations in alternative splicing in dengue-infected human cells. The transcript of the SAT1 gene, of well-known antiviral action, displayed higher inclusion of exon 4 in infected cells, leading to an mRNA isoform that is degraded by non-sense mediated decay. SAT1 is a spermidine/spermine acetyl-transferase enzyme that decreases the reservoir of cellular polyamines, limiting viral replication. Delving into the molecular mechanism underlying SAT1 pre-mRNA splicing changes upon viral infection, we observed lower protein levels of RBM10, a splicing factor responsible for SAT1 exon 4 skipping. We found that the dengue polymerase NS5 interacts with RBM10 and its sole expression triggers RBM10 proteasome-mediated degradation. RBM10 over-expression in infected cells prevents SAT1 splicing changes and limits viral replication, while its knock-down enhances the splicing switch and also benefits viral replication, revealing an anti-viral role for RBM10. Consistently, RBM10 depletion attenuates expression of interferon and pro-inflammatory cytokines. In particular, we found that RBM10 interacts with viral RNA and RIG-I, and even promotes the ubiquitination of the latter, a crucial step for its activation. We propose RBM10 fulfills diverse pro-inflammatory, anti-viral tasks, besides its well-documented role in splicing regulation of apoptotic genes.","journal":"Nucleic Acids Research","year":2020,"id":56982,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":81,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9509,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":297186,"name":"Laureano Bragado","orcid":"0000-0003-4509-9449","position":1,"is_corresponding":false},{"id":297187,"name":"Pablo Mammi","orcid":"0000-0002-3645-531X","position":2,"is_corresponding":false},{"id":297188,"name":"María Florencia Torti","orcid":"0009-0004-0294-3085","position":3,"is_corresponding":false},{"id":299831,"name":"Nicolás Gaioli","orcid":null,"position":4,"is_corresponding":false},{"id":297189,"name":"Leopoldo G. Gebhard","orcid":"0009-0005-5916-0943","position":5,"is_corresponding":false},{"id":297190,"name":"Martín E. García Solá","orcid":"0000-0002-0229-8870","position":6,"is_corresponding":false},{"id":299832,"name":"Rita Vaz‐Drago","orcid":null,"position":7,"is_corresponding":false},{"id":297191,"name":"Néstor Gabriel Iglesias","orcid":"0000-0003-4378-4100","position":8,"is_corresponding":false},{"id":297192,"name":"Cybele C. Garcı́a","orcid":"0000-0002-6431-7176","position":9,"is_corresponding":false},{"id":297193,"name":"Andrea V. Gamarnik","orcid":"0000-0002-9789-4105","position":10,"is_corresponding":false},{"id":297194,"name":"Anabella Srebrow","orcid":"0000-0002-1468-4736","position":11,"is_corresponding":false},{"id":297185,"name":"Berta Pozzi","orcid":"0000-0002-4744-9337","position":0,"is_corresponding":true}],"reference_count":66,"raw_metadata":null,"created_at":"2026-07-18T21:06:31.136481Z","pmid":"32432721","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}