{"doi":"10.1093/nar/gkaa1293","title":"RPA complexes in <i>Caenorhabditis elegans</i> meiosis; unique roles in replication, meiotic recombination and apoptosis","abstract":"Replication Protein A (RPA) is a critical complex that acts in replication and promotes homologous recombination by allowing recombinase recruitment to processed DSB ends. Most organisms possess three RPA subunits (RPA1, RPA2, RPA3) that form a trimeric complex critical for viability. The Caenorhabditis elegans genome encodes RPA-1, RPA-2 and an RPA-2 paralog RPA-4. In our analysis, we determined that RPA-2 is critical for germline replication and normal repair of meiotic DSBs. Interestingly, RPA-1 but not RPA-2 is essential for somatic replication, in contrast to other organisms that require both subunits. Six different hetero- and homodimeric complexes containing permutations of RPA-1, RPA-2 and RPA-4 can be detected in whole animal extracts. Our in vivo studies indicate that RPA-1/4 dimer is less abundant in the nucleus and its formation is inhibited by RPA-2. While RPA-4 does not participate in replication or recombination, we find that RPA-4 inhibits RAD-51 filament formation and promotes apoptosis of a subset of damaged nuclei. Altogether these findings point to sub-functionalization and antagonistic roles of RPA complexes in C. elegans.","journal":"Nucleic Acids Research","year":2020,"id":68847,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9557,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":278939,"name":"Masayoshi Honda","orcid":"0000-0001-8920-6301","position":1,"is_corresponding":false},{"id":364634,"name":"Nicholas M. Cronin","orcid":"0000-0002-9606-5815","position":2,"is_corresponding":false},{"id":365660,"name":"Kailey Harrell","orcid":null,"position":3,"is_corresponding":false},{"id":364635,"name":"Pooja Patel","orcid":"0000-0002-5345-0158","position":4,"is_corresponding":false},{"id":273535,"name":"Maria Spies","orcid":"0000-0002-7375-8037","position":5,"is_corresponding":false},{"id":364636,"name":"Sarit Smolikove","orcid":"0000-0003-0062-3606","position":6,"is_corresponding":false},{"id":364633,"name":"Adam Hefel","orcid":"0000-0002-2135-0152","position":0,"is_corresponding":true}],"reference_count":75,"raw_metadata":null,"created_at":"2026-07-18T21:41:52.279368Z","pmid":"33476370","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}