{"doi":"10.1093/nar/28.5.1145","title":"Regulation of double-strand break-induced mammalian homologous recombination by UBL1, a RAD51-interacting protein","abstract":null,"journal":"Nucleic Acids Research","year":2000,"id":46652,"datarank":4.064810613561138,"base_score":4.276666119016055,"endowment":4.276666119016055,"self_citation_contribution":0.6414999178524083,"citation_network_contribution":3.42331069570873,"self_endowment_contribution":0.6414999178524083,"citer_contribution":3.42331069570873,"corpus_percentile":null,"corpus_rank":null,"citation_count":71,"citer_count":71,"citers_with_citation_signal":64,"citers_with_endowment":64,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":191497,"name":"W. Li","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Regulation of double-strand break-induced mammalian homologous recombination by UBL1, a RAD51-interacting protein","abstract":"Mammalian RAD51 protein plays essential roles in DNA homologous recombination, DNA repair and cell proliferation. RAD51 activities are regulated by its associated proteins. It was previously reported that a ubiquitin-like protein, UBL1, associates with RAD51 in the yeast two-hybrid system. One function of UBL1 is to covalently conjugate with target proteins and thus modify their function. In the present study we found that non-conjugated UBL1 forms a complex with RAD51 and RAD52 proteins in human cells. Overexpression of UBL1 down-regulates DNA double-strand break-induced homologous recombination in CHO cells and reduces cellular resistance to ionizing radiation in HT1080 cells. With or without overexpressed UBL1, most homologous recombination products arise by gene conversion. However, overexpression of UBL1 reduces the fraction of bidirectional gene conversion tracts. Overexpression of a mutant UBL1 that is incapable of being conjugated retains the ability to inhibit homologous recombination. These results suggest a regulatory role for UBL1 in homologous recombination.","is_dataset_classified":null,"base_score":4.276666119016055,"endowment":4.276666119016055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10666456","pmcid":"PMC102610","openalex_id":"https://openalex.org/W2171353009","authors":[],"funders":[{"funder_name":"NIEHS NIH HHS","grant_id":"ES08353","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R37 CA050519","title":null},{"funder_name":"NCI NIH HHS","grant_id":"CA54079","title":null},{"funder_name":"NIEHS NIH HHS","grant_id":"R29 ES008353","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA077693","title":null},{"funder_name":"NCI NIH HHS","grant_id":"CA77693","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA050519","title":null},{"funder_name":"NIEHS NIH HHS","grant_id":"R01 ES008353","title":null}],"total_grants":8,"fwci":3.0678,"citation_percentile":0.91923839,"influential_citations":4,"citation_trend":[{"year":2012,"count":3},{"year":2013,"count":2},{"year":2014,"count":2},{"year":2015,"count":1},{"year":2016,"count":1},{"year":2017,"count":2},{"year":2019,"count":1},{"year":2021,"count":1},{"year":2022,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://academic.oup.com/nar/article-pdf/28/5/1145/9904986/281145.pdf","host_type":"journal"},{"url":"https://academic.oup.com/nar/article-pdf/28/5/1145/9904986/281145.pdf","host_type":"BRONZE"},{"url":"https://academic.oup.com/nar/article-pdf/28/5/1145/9904986/281145.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/nar/article-pdf/28/5/1145/9904986/281145.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/nar/28.5.1145","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/10666456","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/102610","host_type":"repository"}],"fields_of_study":["DNA Repair Mechanisms","Ubiquitin and proteasome pathways","Endoplasmic Reticulum Stress and Disease","Medicine","Biology","Animals","CHO Cells","Cricetinae","DNA","DNA Repair","DNA-Binding Proteins","Humans","Rad51 Recombinase","Recombination, Genetic","SUMO-1 Protein","Transfection","Ubiquitins"],"mesh_terms":["Animals","DNA","DNA Repair","DNA-Binding Proteins","Cricetinae","Humans","Recombination, Genetic","Transfection","Ubiquitins","CHO Cells","SUMO-1 Protein","Rad51 Recombinase"],"keywords":["RAD51","RAD52","Homologous recombination","Biology","Non-homologous end joining","DNA repair protein XRCC4","Homology directed repair","FLP-FRT recombination","Molecular biology","Replication protein A","DNA repair","DNA","Homologous chromosome","Genetic recombination","Gene","Cell biology","DNA-binding protein","Genetics","Recombination","Nucleotide excision repair","Transcription factor"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-12T06:51:04.139350Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}