{"doi":"10.1093/nar/27.14.2912","title":"Synthetic substrate analogs for the RNA-editing adenosine deaminase ADAR-2","abstract":null,"journal":"Nucleic Acids Research","year":1999,"id":619428,"datarank":0.5806801516361837,"base_score":3.8712010109078907,"endowment":3.8712010109078907,"self_citation_contribution":0.5806801516361837,"citation_network_contribution":0.0,"self_endowment_contribution":0.5806801516361837,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":47,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1598495,"name":"H. Yi-Brunozzi","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Synthetic substrate analogs for the RNA-editing adenosine deaminase ADAR-2","abstract":"We have synthesized structural analogs of a natural RNA editing substrate and compared editing reactions of these substrates by recombinant ADAR-2, an RNA-editing adenosine deaminase. Deamination rates were shown to be sensitive to structural changes at the 2[prime]-carbon of the edited adenosine. Methylation of the 2[prime]-OH caused a large decrease in deamination rate, whereas 2[prime]-deoxyadenosine and 2[prime]-deoxy-2[prime]-fluoroadenosine were deaminated at a rate similar to adenosine. In addition, a duplex containing as few as 19 bp of the stem structure adjacent to the R/G editing site of the GluR-B pre-mRNA supports deamination of the R/G adenosine by ADAR-2. This identification and initial characterization of synthetic RNA editing substrate analogs further defines structural elements in the RNA that are important for the deamination reaction and sets the stage for additional detailed structural, thermodynamic and kinetic studies of the ADAR-2 reaction.","is_dataset_classified":null,"base_score":3.8712010109078907,"endowment":3.8712010109078907,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10390533","pmcid":"PMC148506","openalex_id":"https://openalex.org/W2042071572","authors":[],"funders":[],"total_grants":0,"fwci":0.9074,"citation_percentile":0.72423657,"influential_citations":0,"citation_trend":[{"year":2012,"count":3},{"year":2013,"count":1},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2017,"count":5},{"year":2018,"count":2},{"year":2019,"count":2},{"year":2020,"count":1},{"year":2021,"count":2},{"year":2022,"count":3},{"year":2023,"count":2}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://academic.oup.com/nar/article-pdf/27/14/2912/3988499/27-14-2912.pdf","host_type":"journal"},{"url":"https://academic.oup.com/nar/article-pdf/27/14/2912/3988499/27-14-2912.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/nar/article-pdf/27/14/2912/3988499/27-14-2912.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/nar/27.14.2912","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/10390533","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC148506","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/148506","host_type":"repository"}],"fields_of_study":["RNA regulation and disease","RNA Research and Splicing","CRISPR and Genetic Engineering","Adenosine","Adenosine Deaminase","Animals","Base Pairing","Base Sequence","Kinetics","Methylation","Molecular Weight","Nucleic Acid Heteroduplexes","Oligoribonucleotides","RNA Editing","RNA Precursors","RNA, Double-Stranded","RNA-Binding Proteins","Rats","Receptors, AMPA","Recombinant Proteins","Regulatory Sequences, Nucleic Acid","Ribose"],"mesh_terms":["Adenosine","Adenosine Deaminase","Animals","Base Sequence","Kinetics","Methylation","Molecular Weight","Nucleic Acid Heteroduplexes","Oligoribonucleotides","Recombinant Proteins","Regulatory Sequences, Nucleic Acid","Ribose","RNA Precursors","RNA, Double-Stranded","RNA-Binding Proteins","RNA Editing","Receptors, AMPA","Base Pairing","Rats"],"keywords":["ADAR","Deamination","RNA editing","Adenosine deaminase","Biology","RNA","Adenosine","Biochemistry","Deoxyadenosine","Stereochemistry","Enzyme","Gene","Chemistry"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T07:08:22.574135Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}