{"doi":"10.1093/nar/24.15.3107","title":"Phosphorylation of human replication protein A by the DNA-dependent protein kinase is involved in the modulation of DNA replication","abstract":null,"journal":"Nucleic Acids Research","year":1996,"id":589170,"datarank":3.3432427312315154,"base_score":4.02535169073515,"endowment":4.02535169073515,"self_citation_contribution":0.6038027536102726,"citation_network_contribution":2.7394399776212426,"self_endowment_contribution":0.6038027536102726,"citer_contribution":2.7394399776212426,"corpus_percentile":null,"corpus_rank":null,"citation_count":55,"citer_count":55,"citers_with_citation_signal":53,"citers_with_endowment":53,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1507390,"name":"L. Henricksen","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Phosphorylation of human replication protein A by the DNA-dependent protein kinase is involved in the modulation of DNA replication","abstract":"The single-stranded DNA-binding protein, Replication Protein A (RPA), is a heterotrimeric complex with subunits of 70, 32 and 14 kDa involved in DNA metabolism. RPA may be a target for cellular regulation; the 32 kDa subunit (RPA32) is phosphorylated by several cellular kinases including the DNA-dependent protein kinase (DNA-PK). We have purified a mutant hRPA complex lacking amino acids 1-33 of RPA32 (rhRPA x 32delta1-33). This mutant bound ssDNA and supported DNA replication; however, rhRPA x 32delta1-33 was not phosphorylated under replication conditions or directly by DNA-PK. Proteolytic mapping revealed that all the sites phosphorylated by DNA-PK are contained on residues 1-33 of RPA32. When wild-type RPA was treated with DNA-PK and the mixture added to SV40 replication assays, DNA replication was supported. In contrast, when rhRPA x 32delta1-33 was treated with DNA-PK, DNA replication was strongly inhibited. Because untreated rhRPA x 32delta1-33 is fully functional, this suggests that the N-terminus of RPA is needed to overcome inhibitory effects of DNA-PK on other components of the DNA replication system. Thus, phosphorylation of RPA may modulate DNA replication indirectly, through interactions with other proteins whose activity is modulated by phosphorylation.","is_dataset_classified":null,"base_score":4.02535169073515,"endowment":4.02535169073515,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8760901","pmcid":"PMC146026","openalex_id":"https://openalex.org/W2008783898","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM44721","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM64499","title":null}],"total_grants":2,"fwci":2.1856,"citation_percentile":0.88054173,"influential_citations":3,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":2},{"year":2015,"count":1},{"year":2019,"count":1},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://academic.oup.com/nar/article-pdf/24/15/3107/3995329/24-15-3107.pdf","host_type":"journal"},{"url":"https://academic.oup.com/nar/article-pdf/24/15/3107/3995329/24-15-3107.pdf","host_type":"BRONZE"},{"url":"https://academic.oup.com/nar/article-pdf/24/15/3107/3995329/24-15-3107.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/nar/24.15.3107","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8760901","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/146026","host_type":"repository"}],"fields_of_study":["DNA Repair Mechanisms","Mitochondrial Function and Pathology","Genetic Neurodegenerative Diseases","Medicine","Biology"],"mesh_terms":["DNA Replication","DNA-Binding Proteins","DNA, Single-Stranded","DNA, Viral","Humans","Mutation","Nuclear Proteins","Phosphorylation","Protein Binding","Protein Conformation","Structure-Activity Relationship","Simian virus 40","Protein Serine-Threonine Kinases","Sequence Deletion","Replication Protein A","DNA-Activated Protein Kinase"],"keywords":["Replication protein A","Replication factor C","Origin recognition complex","Biology","Eukaryotic DNA replication","Control of chromosome duplication","DNA replication","Ter protein","Pre-replication complex","DNA replication factor CDT1","DNA clamp","Molecular biology","SeqA protein domain","DNA polymerase delta","DNA polymerase II","HMG-box","Cell biology","DNA","Biochemistry","DNA-binding protein","Transcription factor","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-23T14:09:16.152984Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}