{"doi":"10.1093/nar/23.21.4312","title":"A novel enzymatic pathway leading to 1-methylinosine modification in<i>Haloferax volcanii</i>tRNA","abstract":null,"journal":"Nucleic Acids Research","year":1995,"id":673860,"datarank":0.6141516843333151,"base_score":4.0943445622221,"endowment":4.0943445622221,"self_citation_contribution":0.6141516843333151,"citation_network_contribution":0.0,"self_endowment_contribution":0.6141516843333151,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":59,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1760630,"name":"F. Constantinesco","orcid":null,"position":1,"is_corresponding":false},{"id":1760632,"name":"D. Foiret","orcid":null,"position":2,"is_corresponding":false},{"id":1760633,"name":"N. Benachenhou","orcid":null,"position":3,"is_corresponding":false},{"id":1608178,"name":"H. Grosjean","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A novel enzymatic pathway leading to 1-methylinosine modification in<i>Haloferax volcanii</i>tRNA","abstract":"Transfer RNAs of the extreme halophile Haloferax volcanii contain several modified nucleosides, among them 1-methylpseudouridine (m1 psi), pseudouridine (psi), 2'-0-methylcytosine (Cm) and 1-methylinosine (m1l), present in positions 54, 55, 56 and 57 of the psi-loop, respectively. At the same positions in tRNAs from eubacteria and eukaryotes, ribothymidine (T-54), pseudouridine (psi-55), non-modified cytosine (C-56) and non-modified adenosine or guanosine (A-57 or G-57) are found in the so-called T psi-loop. Using as substrate a T7 transcript of Haloferax volcanii tRNA(Ile) devoid of modified nucleosides, the enzymatic activities of several tRNA modification enzymes, including those for m1 psi-54, psi-55, Cm-56 and m1l-57, were detected in cell extracts of H.volcanii. Here, we demonstrate that modification of A-57 into m1l-57 in H.volcanii tRNA(Ile) occurs via a two-step enzymatic process. The first step corresponds to the formation of m1A-57 catalyzed by a S-adenosylmethionine-dependent tRNA methyltransferase, followed by the deamination of the 6-amino group of the adenine moiety by a 1-methyladenosine-57 deaminase. This enzymatic pathway differs from that leading to the formation of m1l-37 in the anticodon loop of eukaryotic tRNA(Ala). In the latter case, inosine-37 formation preceeds the S-adenosylmethionine-dependent methylation of l-37 into m1l-37. Thus, enzymatic strategies for catalyzing the formation of 1-methylinosine in tRNAs differ in organisms from distinct evolutionary kingdoms.","is_dataset_classified":null,"base_score":4.0943445622221,"endowment":4.0943445622221,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7501451","pmcid":"PMC307385","openalex_id":"https://openalex.org/W2067699519","authors":[],"funders":[],"total_grants":0,"fwci":0.2605,"citation_percentile":0.514897,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2014,"count":8},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2017,"count":4},{"year":2018,"count":4},{"year":2019,"count":4},{"year":2020,"count":4},{"year":2021,"count":4},{"year":2022,"count":3},{"year":2023,"count":3},{"year":2024,"count":2},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/307385","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/307385","host_type":"repository"},{"url":"http://academic.oup.com/nar/article-pdf/23/21/4312/6169813/23-21-4312.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/nar/23.21.4312","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7501451","host_type":"repository"}],"fields_of_study":["RNA modifications and cancer","RNA and protein synthesis mechanisms","Metal-Organic Frameworks: Synthesis and Applications","Adenosine","Base Sequence","Halobacteriaceae","Inosine","Molecular Sequence Data","Nucleosides","Pseudouridine","RNA Processing, Post-Transcriptional","RNA, Bacterial","RNA, Transfer, Ile","Subcellular Fractions"],"mesh_terms":["Adenosine","Base Sequence","Halobacteriaceae","Inosine","Molecular Sequence Data","Nucleosides","Pseudouridine","RNA Processing, Post-Transcriptional","RNA, Bacterial","RNA, Transfer, Ile","Subcellular Fractions"],"keywords":["Haloferax volcanii","Pseudouridine","Transfer RNA","Biology","Biochemistry","Endoribonuclease","Guanosine","Stereochemistry","Enzyme","Methylation","Inosine","DNA","RNA","Chemistry","Archaea","RNase P"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T14:43:51.810892Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}