{"doi":"10.1093/nar/19.22.6155","title":"High level expression and purification of herpes simplex virus type 1 immediate early polypeptide Vmw110","abstract":null,"journal":"Nucleic Acids Research","year":1991,"id":686319,"datarank":0.6166310796259968,"base_score":4.110873864173311,"endowment":4.110873864173311,"self_citation_contribution":0.6166310796259968,"citation_network_contribution":0.0,"self_endowment_contribution":0.6166310796259968,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":60,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":118349,"name":"Anne Orr","orcid":null,"position":1,"is_corresponding":false},{"id":118347,"name":"Margaret Elliott","orcid":null,"position":2,"is_corresponding":false},{"id":118346,"name":"Roger D. Everett","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"High level expression and purification of herpes simplex virus type 1 immediate early polypeptide Vmw110","abstract":"Herpes simplex virus type 1 (HSV-1) encodes five immediate early (IE) polypeptides. This paper reports the construction of a baculovirus vector which expresses large amounts of Vmw110, the product of IE gene 1. The expressed protein has been purified to near homogeneity and has a mobility on SDS polyacrylamide gels identical to that of Vmw110 produced during HSV-1 infection. Characterisation of its properties indicated that it forms dimers and perhaps higher order oligomers in solution and that the purified protein binds to both single stranded and double stranded calf thymus DNA cellulose columns. However, filter binding experiments were unable to detect any stable association of Vmw110 with DNA in solution.","is_dataset_classified":null,"base_score":4.110873864173311,"endowment":4.110873864173311,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"1659686","pmcid":"PMC329111","openalex_id":"https://openalex.org/W2058416132","authors":[],"funders":[],"total_grants":0,"fwci":2.3029,"citation_percentile":0.87609582,"influential_citations":0,"citation_trend":[{"year":2012,"count":4},{"year":2015,"count":1},{"year":2022,"count":1},{"year":2024,"count":2}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/329111","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/329111","host_type":"repository"},{"url":"http://academic.oup.com/nar/article-pdf/19/22/6155/3316691/19-22-6155.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/nar/19.22.6155","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/1659686","host_type":"repository"},{"url":"http://eprints.gla.ac.uk/view/author/11900.html>,","host_type":"journal"},{"url":"http://europepmc.org/pmc/articles/PMC329111","host_type":"repository"}],"fields_of_study":["Herpesvirus Infections and Treatments","Virus-based gene therapy research","Toxin Mechanisms and Immunotoxins","Animals","Baculoviridae","Blotting, Western","Cattle","Chromatography, Liquid","DNA","DNA-Binding Proteins","Electrophoresis, Polyacrylamide Gel","Enzyme-Linked Immunosorbent Assay","Gene Expression","Genes, Viral","Genetic Vectors","Immediate-Early Proteins","Simplexvirus","Ubiquitin-Protein Ligases","Viral Proteins"],"mesh_terms":["Animals","Cattle","Chromatography, Liquid","DNA","DNA-Binding Proteins","Electrophoresis, Polyacrylamide Gel","Enzyme-Linked Immunosorbent Assay","Genes, Viral","Genetic Vectors","Viral Proteins","Blotting, Western","Gene Expression","Baculoviridae","Immediate-Early Proteins","Simplexvirus","Ubiquitin-Protein Ligases"],"keywords":["Biology","Herpes simplex virus","DNA","Molecular biology","Gene","Single-stranded binding protein","Recombinant DNA","Polyacrylamide gel electrophoresis","Virus","Biochemistry","Plasmid","Oligonucleotide","Gene product","Virology","DNA-binding protein","Gene expression","Enzyme","Transcription factor"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T19:09:32.210789Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}