{"doi":"10.1093/nar/18.4.753","title":"Does butyiphenyl-deoxyguanosine triphosphate differentially inhibit DNA polymerase α and δ activities in permeabilized HeLa cells?","abstract":null,"journal":"Nucleic Acids Research","year":1990,"id":683710,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1218301,"name":"A. Jackson","orcid":"0000-0002-3319-4871","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Does butyiphenyl-deoxyguanosine triphosphate differentially inhibit DNA polymerase α and δ activities in permeabilized HeLa cells?","abstract":"In eukaryotic cells, two enzymes, DNA polymerases alpha and delta, are thought to play major roles in DNA synthesis. I have used butylphenyl dGTP (BuPdGTP), a potent inhibitor of purified DNA polymerase alpha, to assess the relative activities of these enzymes in two permeabilized cell systems. In both instances BuPdGTP eliminated all of the activity which was sensitive to aphidicolin. However, no conditions were found where BuPdGTP preferentially inhibited the synthesis of Okazaki fragments--the presumed products of DNA polymerase alpha activity. This implies that DNA polymerase activities on the two sides of the replication fork are unable to operate independently, being just two elements of the integrated replication machinery that undertakes DNA synthesis in permeabilized cells.","is_dataset_classified":null,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"2315038","pmcid":"PMC330323","openalex_id":"https://openalex.org/W1963656236","authors":[],"funders":[],"total_grants":0,"fwci":0.1457,"citation_percentile":0.42946279,"influential_citations":0,"citation_trend":[{"year":2015,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/330323","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/330323","host_type":"repository"},{"url":"http://academic.oup.com/nar/article-pdf/18/4/753/3882968/18-4-753.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/nar/18.4.753","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/2315038","host_type":"repository"}],"fields_of_study":["DNA Repair Mechanisms","DNA and Nucleic Acid Chemistry","Bacterial Genetics and Biotechnology","Cell Membrane Permeability","DNA Polymerase I","DNA Polymerase II","DNA Replication","Deoxyguanine Nucleotides","HeLa Cells","Humans","Hypertonic Solutions","Kinetics"],"mesh_terms":["Cell Membrane Permeability","Deoxyguanine Nucleotides","DNA Polymerase I","DNA Polymerase II","DNA Replication","HeLa Cells","Humans","Hypertonic Solutions","Kinetics","Hela Cells"],"keywords":["Biology","DNA polymerase","Okazaki fragments","DNA polymerase II","Aphidicolin","DNA clamp","DNA replication","DNA polymerase I","Polymerase","DNA polymerase delta","Molecular biology","DNA synthesis","Biochemistry","DNA","Primase","Eukaryotic DNA replication","Polymerase chain reaction","Gene","Reverse transcriptase"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T11:56:56.988615Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}