{"doi":"10.1093/nar/18.21.6223","title":"Conserved domains of human U4 snRNA required for snRNP and spliceosome assembly","abstract":null,"journal":"Nucleic Acids Research","year":1990,"id":598047,"datarank":0.5456379239589579,"base_score":3.6375861597263857,"endowment":3.6375861597263857,"self_citation_contribution":0.5456379239589579,"citation_network_contribution":0.0,"self_endowment_contribution":0.5456379239589579,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":37,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":179894,"name":"Albrecht Bindereif","orcid":null,"position":1,"is_corresponding":false},{"id":1532282,"name":"Cornelia Wersig","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Conserved domains of human U4 snRNA required for snRNP and spliceosome assembly","abstract":"U4 snRNA is phylogenetically highly conserved and organized in several domains. To determine the function of each of the domains of human U4 snRNA in the multi-step process of snRNP and spliceosome assembly, we used reconstitution procedures in combination with snRNA mutagenesis. The highly conserved 5' terminal domain of U4 snRNA consists of the stem I and stem II regions that have been proposed to base pair with U6 snRNA, and the 5' stem-loop structure. We found that each of these structural elements is essential for spliceosome assembly. However, only the stem II region is required for U4-U6 interaction, and none of these elements for Sm protein binding. In contrast, the 3' terminal domain of U4 snRNA containing the Sm binding site is dispensable for both U4-U6 interaction and spliceosome assembly. Our results support an organization of the U4 snRNP into multiple functional domains, each of which acts at distinct stages of snRNP and spliceosome assembly.","is_dataset_classified":null,"base_score":3.6375861597263857,"endowment":3.6375861597263857,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"2147057","pmcid":"PMC332485","openalex_id":"https://openalex.org/W2025071510","authors":[],"funders":[],"total_grants":0,"fwci":1.6429,"citation_percentile":0.84077932,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2014,"count":1},{"year":2022,"count":1},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/332485","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/332485","host_type":"repository"},{"url":"http://academic.oup.com/nar/article-pdf/18/21/6223/7078312/18-21-6223.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1093/nar/18.21.6223","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/2147057","host_type":"repository"},{"url":"http://europepmc.org/articles/PMC332485","host_type":"repository"}],"fields_of_study":["RNA Research and Splicing","RNA and protein synthesis mechanisms","RNA modifications and cancer"],"mesh_terms":["Base Sequence","Chromosome Deletion","Humans","Molecular Sequence Data","Nucleic Acid Conformation","Phylogeny","Ribonucleoproteins","RNA Splicing","RNA, Small Nuclear","Restriction Mapping","Oligonucleotide Probes","Mutagenesis, Site-Directed","Ribonucleoproteins, Small Nuclear"],"keywords":["Prp24","snRNP","Small nuclear RNA","Spliceosome","Biology","Cell biology","Genetics","RNA splicing","RNA","Non-coding RNA","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T14:56:03.828476Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}